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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F14%3A00433745%21RIV15-GA0-61389030
rdf:type
n13:Vysledek skos:Concept
rdfs:seeAlso
http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=25117271
dcterms:description
FM (Fei-Mao) styryl dyes are compounds of amphiphilic character that are used for the fluorescence tracking of endocytosis and related processes, i.e., the internalization of membrane vesicles from the plasma membrane (PM) and dynamics of endomembranes. Staining with FM dyes and subsequent microscopical observations could be performed both on the tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and de-staining in root epidermal cells of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells. The progression of FM dye uptake, reflected by an increased amount of the dye in the endosomal compartments, is monitored under the fluorescence microscope in a time-lapse manner. The data obtained can be used for the characterization of the rate of endocytosis and the function of components of endosomal recycling machinery. FM (Fei-Mao) styryl dyes are compounds of amphiphilic character that are used for the fluorescence tracking of endocytosis and related processes, i.e., the internalization of membrane vesicles from the plasma membrane (PM) and dynamics of endomembranes. Staining with FM dyes and subsequent microscopical observations could be performed both on the tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and de-staining in root epidermal cells of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells. The progression of FM dye uptake, reflected by an increased amount of the dye in the endosomal compartments, is monitored under the fluorescence microscope in a time-lapse manner. The data obtained can be used for the characterization of the rate of endocytosis and the function of components of endosomal recycling machinery.
dcterms:title
The use of FM dyes to analyze plant endocytosis The use of FM dyes to analyze plant endocytosis
skos:prefLabel
The use of FM dyes to analyze plant endocytosis The use of FM dyes to analyze plant endocytosis
skos:notation
RIV/61389030:_____/14:00433745!RIV15-GA0-61389030
n5:aktivita
n6:I n6:P
n5:aktivity
I, P(GAP305/11/2476), P(GPP305/11/P797)
n5:dodaniDat
n19:2015
n5:domaciTvurceVysledku
n8:5790662 n8:2055589 n8:5408954
n5:druhVysledku
n20:C
n5:duvernostUdaju
n14:S
n5:entitaPredkladatele
n21:predkladatel
n5:idSjednocenehoVysledku
52284
n5:idVysledku
RIV/61389030:_____/14:00433745
n5:jazykVysledku
n18:eng
n5:klicovaSlova
Endocytosis; Endomembranes; FM 1-43
n5:klicoveSlovo
n11:FM%201-43 n11:Endocytosis n11:Endomembranes
n5:kontrolniKodProRIV
[F63E224340BF]
n5:mistoVydani
Totowa
n5:nazevEdiceCisloSvazku
Methods in Molecular Biology
n5:nazevZdroje
Plant Endosomes - Methods and Protocols
n5:obor
n7:EB
n5:pocetDomacichTvurcuVysledku
3
n5:pocetStranKnihy
283
n5:pocetTvurcuVysledku
3
n5:projekt
n12:GAP305%2F11%2F2476 n12:GPP305%2F11%2FP797
n5:rokUplatneniVysledku
n19:2014
n5:tvurceVysledku
Jelínková, Adriana Malínská, Kateřina Petrášek, Jan
s:numberOfPages
11
n16:doi
10.1007/978-1-4939-1420-3_1
n17:hasPublisher
Humana Press
n15:isbn
978-1-4939-1419-7