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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F14%3A00433240%21RIV15-AV0-61389030
rdf:type
skos:Concept n15:Vysledek
dcterms:description
The trap of the carnivorous plant Venus flytrap (Dionaea muscipula) catches prey by very rapid closure of its modified leaves. After the rapid closure secures the prey, repeated mechanical stimulation of trigger hairs by struggling prey and the generation of action potentials (APs) result in secretion of digestive fluid. Once the prey's movement stops, the secretion is maintained by chemical stimuli released from digested prey. We investigated the effect of mechanical and chemical stimulation (NH4Cl, KH2PO4, further N(Cl) and P(K) stimulation) on enzyme activities in digestive fluid. Activities of beta-D-glucosidases and N-acetyl-beta-D-glucosaminidases were not detected. Acid phosphatase activity was higher in N(Cl) stimulated traps while proteolytic activity was higher in both chemically induced traps in comparison to mechanical stimulation. This is in accordance with higher abundance of recently described enzyme cysteine endopeptidase dionain in digestive fluid of chemically induced traps. Mechanical stimulation induced high levels of cis-12-oxophytodienoic acid (cis-OPDA) but jasmonic acid (JA) and its isoleucine conjugate (JA-Ile) accumulated to higher level after chemical stimulation. The concentration of indole-3-acetic acid (IAA), salicylic acid (SA) and abscisic acid (ABA) did not change significantly. The external application of JA bypassed the mechanical and chemical stimulation and induced a high abundance of dionain and proteolytic activity in digestive fluid. These results document the role of jasmonates in regulation of proteolytic activity in response to different stimuli from captured prey. The double trigger mechanism in protein digestion is proposed. The trap of the carnivorous plant Venus flytrap (Dionaea muscipula) catches prey by very rapid closure of its modified leaves. After the rapid closure secures the prey, repeated mechanical stimulation of trigger hairs by struggling prey and the generation of action potentials (APs) result in secretion of digestive fluid. Once the prey's movement stops, the secretion is maintained by chemical stimuli released from digested prey. We investigated the effect of mechanical and chemical stimulation (NH4Cl, KH2PO4, further N(Cl) and P(K) stimulation) on enzyme activities in digestive fluid. Activities of beta-D-glucosidases and N-acetyl-beta-D-glucosaminidases were not detected. Acid phosphatase activity was higher in N(Cl) stimulated traps while proteolytic activity was higher in both chemically induced traps in comparison to mechanical stimulation. This is in accordance with higher abundance of recently described enzyme cysteine endopeptidase dionain in digestive fluid of chemically induced traps. Mechanical stimulation induced high levels of cis-12-oxophytodienoic acid (cis-OPDA) but jasmonic acid (JA) and its isoleucine conjugate (JA-Ile) accumulated to higher level after chemical stimulation. The concentration of indole-3-acetic acid (IAA), salicylic acid (SA) and abscisic acid (ABA) did not change significantly. The external application of JA bypassed the mechanical and chemical stimulation and induced a high abundance of dionain and proteolytic activity in digestive fluid. These results document the role of jasmonates in regulation of proteolytic activity in response to different stimuli from captured prey. The double trigger mechanism in protein digestion is proposed.
dcterms:title
Abundance of Cysteine Endopeptidase Dionain in Digestive Fluid of Venus Flytrap (Dionaea muscipula Ellis) Is Regulated by Different Stimuli from Prey through Jasmonates Abundance of Cysteine Endopeptidase Dionain in Digestive Fluid of Venus Flytrap (Dionaea muscipula Ellis) Is Regulated by Different Stimuli from Prey through Jasmonates
skos:prefLabel
Abundance of Cysteine Endopeptidase Dionain in Digestive Fluid of Venus Flytrap (Dionaea muscipula Ellis) Is Regulated by Different Stimuli from Prey through Jasmonates Abundance of Cysteine Endopeptidase Dionain in Digestive Fluid of Venus Flytrap (Dionaea muscipula Ellis) Is Regulated by Different Stimuli from Prey through Jasmonates
skos:notation
RIV/61389030:_____/14:00433240!RIV15-AV0-61389030
n3:aktivita
n11:S n11:P n11:I
n3:aktivity
I, P(EE2.3.20.0057), P(LO1204), S
n3:cisloPeriodika
8
n3:dodaniDat
n4:2015
n3:domaciTvurceVysledku
n6:3686957 n6:7549261
n3:druhVysledku
n13:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
1375
n3:idVysledku
RIV/61389030:_____/14:00433240
n3:jazykVysledku
n14:eng
n3:klicovaSlova
PLANT NEPENTHES-ALATA; SECRETORY CYCLE; PHOTOSYNTHETIC EFFICIENCY
n3:klicoveSlovo
n17:SECRETORY%20CYCLE n17:PLANT%20NEPENTHES-ALATA n17:PHOTOSYNTHETIC%20EFFICIENCY
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[45D72C45F2C6]
n3:nazevZdroje
PLoS ONE
n3:obor
n10:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
5
n3:projekt
n7:EE2.3.20.0057 n7:LO1204
n3:rokUplatneniVysledku
n4:2014
n3:svazekPeriodika
9
n3:tvurceVysledku
Slováková, L. Floková, Kristýna Libiaková, M. Novák, Ondřej Pavlovič, A.
n3:wos
000340952200019
s:issn
1932-6203
s:numberOfPages
12
n18:doi
10.1371/journal.pone.0104424