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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F14%3A00432723%21RIV15-GA0-61389030
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Phospholipids have recently been found to be integral elements of hormone signalling pathways. An Arabidopsis thaliana double mutant in two type III phosphatidylinositol-4-kinases (PI4Ks), pi4kIII beta 1 beta 2, displays a stunted rosette growth. The causal link between PI4K activity and growth is unknown. Using microarray analysis, quantitative reverse transcription polymerase chain reaction (RT-qPCR) and multiple phytohormone analysis by LC-MS we investigated the mechanism responsible for the pi4kIII beta 1 beta 2 phenotype. The pi4kIII beta 1 beta 2 mutant accumulated a high concentration of salicylic acid (SA), constitutively expressed SA marker genes including PR-1, and was more resistant to Pseudomonas syringae. pi4kIII beta 1 beta 2 was crossed with SA signalling mutants eds1 and npr1 and SA biosynthesis mutant sid2 and NahG. The dwarf phenotype of pi4kIII beta 1 beta 2 rosettes was suppressed in all four triple mutants. Whereas eds1 pi4kIII beta 1 beta 2, sid2 pi4kIII beta 1 beta 2 and NahG pi4kIII beta 1 beta 2 had similar amounts of SA as the wild-type (WT), npr1pi4kIII beta 1 beta 2 had more SA than pi4kIII beta 1 beta 2 despite being less dwarfed. This indicates that PI4KIII beta 1 and PI4KIII beta 2 are genetically upstream of EDS1 and need functional SA biosynthesis and perception through NPR1 to express the dwarf phenotype. The slow root growth phenotype of pi4kIII beta 1 beta 2 was not suppressed in any of the triple mutants. The pi4kIII beta 1 beta 2 mutations together cause constitutive activation of SA signalling that is responsible for the dwarf rosette phenotype but not for the short root phenotype. Phospholipids have recently been found to be integral elements of hormone signalling pathways. An Arabidopsis thaliana double mutant in two type III phosphatidylinositol-4-kinases (PI4Ks), pi4kIII beta 1 beta 2, displays a stunted rosette growth. The causal link between PI4K activity and growth is unknown. Using microarray analysis, quantitative reverse transcription polymerase chain reaction (RT-qPCR) and multiple phytohormone analysis by LC-MS we investigated the mechanism responsible for the pi4kIII beta 1 beta 2 phenotype. The pi4kIII beta 1 beta 2 mutant accumulated a high concentration of salicylic acid (SA), constitutively expressed SA marker genes including PR-1, and was more resistant to Pseudomonas syringae. pi4kIII beta 1 beta 2 was crossed with SA signalling mutants eds1 and npr1 and SA biosynthesis mutant sid2 and NahG. The dwarf phenotype of pi4kIII beta 1 beta 2 rosettes was suppressed in all four triple mutants. Whereas eds1 pi4kIII beta 1 beta 2, sid2 pi4kIII beta 1 beta 2 and NahG pi4kIII beta 1 beta 2 had similar amounts of SA as the wild-type (WT), npr1pi4kIII beta 1 beta 2 had more SA than pi4kIII beta 1 beta 2 despite being less dwarfed. This indicates that PI4KIII beta 1 and PI4KIII beta 2 are genetically upstream of EDS1 and need functional SA biosynthesis and perception through NPR1 to express the dwarf phenotype. The slow root growth phenotype of pi4kIII beta 1 beta 2 was not suppressed in any of the triple mutants. The pi4kIII beta 1 beta 2 mutations together cause constitutive activation of SA signalling that is responsible for the dwarf rosette phenotype but not for the short root phenotype.
dcterms:title
Constitutive salicylic acid accumulation in pi4kIII beta 1 beta 2 Arabidopsis plants stunts rosette but not root growth Constitutive salicylic acid accumulation in pi4kIII beta 1 beta 2 Arabidopsis plants stunts rosette but not root growth
skos:prefLabel
Constitutive salicylic acid accumulation in pi4kIII beta 1 beta 2 Arabidopsis plants stunts rosette but not root growth Constitutive salicylic acid accumulation in pi4kIII beta 1 beta 2 Arabidopsis plants stunts rosette but not root growth
skos:notation
RIV/61389030:_____/14:00432723!RIV15-GA0-61389030
n5:aktivita
n18:P n18:I
n5:aktivity
I, P(GAP501/11/1654)
n5:cisloPeriodika
3
n5:dodaniDat
n9:2015
n5:domaciTvurceVysledku
n6:5885884 n6:5751691 n6:4739841 n6:3469689 n6:4495349
n5:druhVysledku
n15:J
n5:duvernostUdaju
n14:S
n5:entitaPredkladatele
n16:predkladatel
n5:idSjednocenehoVysledku
8676
n5:idVysledku
RIV/61389030:_____/14:00432723
n5:jazykVysledku
n7:eng
n5:klicovaSlova
Arabidopsis; dwarf phenotype; hormone transduction
n5:klicoveSlovo
n11:dwarf%20phenotype n11:Arabidopsis n11:hormone%20transduction
n5:kodStatuVydavatele
US - Spojené státy americké
n5:kontrolniKodProRIV
[ECF597DD0850]
n5:nazevZdroje
New Phytologist
n5:obor
n13:CE
n5:pocetDomacichTvurcuVysledku
5
n5:pocetTvurcuVysledku
13
n5:projekt
n10:GAP501%2F11%2F1654
n5:rokUplatneniVysledku
n9:2014
n5:svazekPeriodika
203
n5:tvurceVysledku
Maseda, Encarnación López Puyaubert, J. Guivarc'h, A. Caius, J. Dobrev, Petre Janda, Martin Valentová, O. Zachowski, A. Delage, E. Ruelland, E. Burketová, Lenka Šašek, Vladimír
n5:wos
000339556300011
s:issn
0028-646X
s:numberOfPages
12
n17:doi
10.1111/nph.12822