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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F14%3A00430550%21RIV15-GA0-61389030
rdf:type
n19:Vysledek skos:Concept
dcterms:description
Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application. Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application.
dcterms:title
Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters
skos:prefLabel
Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters
skos:notation
RIV/61389030:_____/14:00430550!RIV15-GA0-61389030
n3:aktivita
n12:P n12:I
n3:aktivity
I, P(GAP305/11/0797)
n3:dodaniDat
n6:2015
n3:domaciTvurceVysledku
n8:5465222 n8:9070796 n8:5408954 n8:2027178 n8:4739841
n3:druhVysledku
n17:C
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
3224
n3:idVysledku
RIV/61389030:_____/14:00430550
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Auxin; Auxin metabolism; BY-2 tobacco cells
n3:klicoveSlovo
n4:BY-2%20tobacco%20cells n4:Auxin n4:Auxin%20metabolism
n3:kontrolniKodProRIV
[14E4CA2D26B3]
n3:mistoVydani
Totowa
n3:nazevEdiceCisloSvazku
Methods in Molecular Biology
n3:nazevZdroje
PLANT CHEMICAL GENOMICS: METHODS AND PROTOCOLS
n3:obor
n9:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetStranKnihy
269
n3:pocetTvurcuVysledku
6
n3:projekt
n16:GAP305%2F11%2F0797
n3:rokUplatneniVysledku
n6:2014
n3:tvurceVysledku
Zažímalová, Eva Dobrev, Petre Petrášek, Jan Skůpa, Petr Simon, Sibu Friml, J.
s:numberOfPages
10
n18:doi
10.1007/978-1-62703-592-7_23
n15:hasPublisher
Humana Press
n20:isbn
978-1-62703-591-0