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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F14%3A00429994%21RIV15-AV0-61389030
rdf:type
n16:Vysledek skos:Concept
dcterms:description
The accumulation of radioactively labelled compounds in cells is frequently used for the determination of activities of various transport systems located at the plasma membrane, including the system for carrier-mediated transport of plant hormone auxin. The measurements of auxin transport could be performed on the tissue level as well, but for more precise quantitative analysis of activity of individual auxin carriers the model of plant cell cultures represents an invaluable tool. Here, we describe the method for the determination of the activities of auxin influx and efflux carriers in plant cells grown in a suspension using radiolabelled synthetic auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene-1-acetic acid (NAA). By making use of specific inhibitors of active auxin influx and efflux, as well as cell lines overexpressing or silencing particular auxin carriers, this method allows the determination of kinetic parameters of auxin flow across the plasma membrane and the activity of those carriers. The accumulation of radioactively labelled compounds in cells is frequently used for the determination of activities of various transport systems located at the plasma membrane, including the system for carrier-mediated transport of plant hormone auxin. The measurements of auxin transport could be performed on the tissue level as well, but for more precise quantitative analysis of activity of individual auxin carriers the model of plant cell cultures represents an invaluable tool. Here, we describe the method for the determination of the activities of auxin influx and efflux carriers in plant cells grown in a suspension using radiolabelled synthetic auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene-1-acetic acid (NAA). By making use of specific inhibitors of active auxin influx and efflux, as well as cell lines overexpressing or silencing particular auxin carriers, this method allows the determination of kinetic parameters of auxin flow across the plasma membrane and the activity of those carriers.
dcterms:title
Determination of Auxin Transport Parameters on the Cellular Level Determination of Auxin Transport Parameters on the Cellular Level
skos:prefLabel
Determination of Auxin Transport Parameters on the Cellular Level Determination of Auxin Transport Parameters on the Cellular Level
skos:notation
RIV/61389030:_____/14:00429994!RIV15-AV0-61389030
n4:aktivita
n13:I
n4:aktivity
I
n4:dodaniDat
n12:2015
n4:domaciTvurceVysledku
n8:2027178 n8:1385925 n8:5408954
n4:druhVysledku
n9:C
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n19:predkladatel
n4:idSjednocenehoVysledku
10737
n4:idVysledku
RIV/61389030:_____/14:00429994
n4:jazykVysledku
n7:eng
n4:klicovaSlova
Auxin transport assay; Plant cell cultures; Auxin influx
n4:klicoveSlovo
n10:Plant%20cell%20cultures n10:Auxin%20influx n10:Auxin%20transport%20assay
n4:kontrolniKodProRIV
[F63B7C77990D]
n4:mistoVydani
Totowa
n4:nazevZdroje
Plant Chemical Genomics
n4:obor
n18:ED
n4:pocetDomacichTvurcuVysledku
3
n4:pocetStranKnihy
269
n4:pocetTvurcuVysledku
3
n4:rokUplatneniVysledku
n12:2014
n4:tvurceVysledku
Zažímalová, Eva Petrášek, Jan Laňková, Martina
s:numberOfPages
13
n5:doi
10.1007/978-1-62703-592-7_22
n17:hasPublisher
Humana Press
n3:isbn
978-1-62703-591-0