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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F14%3A00429992%21RIV15-GA0-61389030
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Increased endogenous plant cytokinin (CK) content through transformation with an isopentyl transferase (ipt) gene has been associated with improved plant stress tolerance. The objective of this study is to determine amino acid changes associated with elevated CK production in ipt transgenic tobacco (Nicotiana tabacum L, cv. Wisconsin 38). Nontransformed (WT) and transformed tobacco plants with ipt gene controlled by senescence-activated promoter (SAG) were exposed to zinc soil contamination (tested levels Zn1=250, Zn2=500, Zn3=750 mg kg(-1) soil). The Zn effect on plant stress metabolism resulted in changes in levels of selected free amino acids playing an important role in adaptation to stress and plant senescence (alanine, leucine, proline, methionine and gamma-aminobutyrate) and differed for transformed and nontransformed tobacco plants. Analyses of amino adds confirmed that SAG tobacco plants had improved zinc tolerance compared with the WT plants. The enhanced Zn tolerance of SAG plants was associated with the maintenance of accumulation of proline, methionine and gamma-aminobutyrate. The concentrations of leucine and alanine did not show significant differences between plant lines. Increased endogenous plant cytokinin (CK) content through transformation with an isopentyl transferase (ipt) gene has been associated with improved plant stress tolerance. The objective of this study is to determine amino acid changes associated with elevated CK production in ipt transgenic tobacco (Nicotiana tabacum L, cv. Wisconsin 38). Nontransformed (WT) and transformed tobacco plants with ipt gene controlled by senescence-activated promoter (SAG) were exposed to zinc soil contamination (tested levels Zn1=250, Zn2=500, Zn3=750 mg kg(-1) soil). The Zn effect on plant stress metabolism resulted in changes in levels of selected free amino acids playing an important role in adaptation to stress and plant senescence (alanine, leucine, proline, methionine and gamma-aminobutyrate) and differed for transformed and nontransformed tobacco plants. Analyses of amino adds confirmed that SAG tobacco plants had improved zinc tolerance compared with the WT plants. The enhanced Zn tolerance of SAG plants was associated with the maintenance of accumulation of proline, methionine and gamma-aminobutyrate. The concentrations of leucine and alanine did not show significant differences between plant lines.
dcterms:title
The long-term effect of zinc soil contamination on selected free amino acids playing an important role in plant adaptation to stress and senescence The long-term effect of zinc soil contamination on selected free amino acids playing an important role in plant adaptation to stress and senescence
skos:prefLabel
The long-term effect of zinc soil contamination on selected free amino acids playing an important role in plant adaptation to stress and senescence The long-term effect of zinc soil contamination on selected free amino acids playing an important role in plant adaptation to stress and senescence
skos:notation
RIV/61389030:_____/14:00429992!RIV15-GA0-61389030
n3:aktivita
n16:P n16:I
n3:aktivity
I, P(GAP501/11/1239)
n3:cisloPeriodika
FEB 2014
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n12:8437483 n12:5014395 n12:9840419
n3:druhVysledku
n18:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
26587
n3:idVysledku
RIV/61389030:_____/14:00429992
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Amino acid; Cytokinins; Senescence
n3:klicoveSlovo
n8:Senescence n8:Amino%20acid n8:Cytokinins
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[FC83A2382B37]
n3:nazevZdroje
Ecotoxicology and Enviromental Safety
n3:obor
n15:EF
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n4:GAP501%2F11%2F1239
n3:rokUplatneniVysledku
n10:2014
n3:svazekPeriodika
100
n3:tvurceVysledku
Száková, J. Pavlíková, D. Zemanová, V. Procházková, Dagmar Pavlík, Milan Wilhelmová, Naděžda
n3:wos
000330161000023
s:issn
0147-6513
s:numberOfPages
5
n14:doi
10.1016/j.ecoenv.2013.10.028