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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F14%3A00429973%21RIV15-AV0-61389030
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Growth regulators and carbohydrates are key regulatory factors that affect somatic embryogenesis. Carbohydrates serve as energy and carbon sources, osmotica and osmoprotectants and are important signal molecules. Most information about the role of carbohydrates in somatic embryogenesis in Norway spruce has been obtained with embryos grown on semi-solid media. The aim of the present study was to gain a better understanding of the effects of exogenous carbohydrates through modification of medium components (sugars) and physical state (liquid and semi-solid media). Rafts, floating on liquid medium, were used to allow precise manipulation of carbohydrate availability, though it did not result in the highest embryo yields. Our results indicate the following for Norway spruce somatic embryo development: (1) overall carbohydrate dynamics in somatic embryos cultivated on liquid or semi-solid media were similar; (2) the total carbohydrate content, however, was higher in somatic embryos cultivated on liquid media; (3) sucrose was present in somatic embryos even when they matured on sucrose-free media; (4) sucrose content in liquid sucrose-supplemented maturation media decreased sharply during a 1-wk subculture interval; (5) the accumulation of the raffinose family oligosaccharides during desiccation was determined independently of previous sugar supply; and (6) a decrease of sucrose and an increase of hexoses contents accompanied somatic embryo germination. Growth regulators and carbohydrates are key regulatory factors that affect somatic embryogenesis. Carbohydrates serve as energy and carbon sources, osmotica and osmoprotectants and are important signal molecules. Most information about the role of carbohydrates in somatic embryogenesis in Norway spruce has been obtained with embryos grown on semi-solid media. The aim of the present study was to gain a better understanding of the effects of exogenous carbohydrates through modification of medium components (sugars) and physical state (liquid and semi-solid media). Rafts, floating on liquid medium, were used to allow precise manipulation of carbohydrate availability, though it did not result in the highest embryo yields. Our results indicate the following for Norway spruce somatic embryo development: (1) overall carbohydrate dynamics in somatic embryos cultivated on liquid or semi-solid media were similar; (2) the total carbohydrate content, however, was higher in somatic embryos cultivated on liquid media; (3) sucrose was present in somatic embryos even when they matured on sucrose-free media; (4) sucrose content in liquid sucrose-supplemented maturation media decreased sharply during a 1-wk subculture interval; (5) the accumulation of the raffinose family oligosaccharides during desiccation was determined independently of previous sugar supply; and (6) a decrease of sucrose and an increase of hexoses contents accompanied somatic embryo germination.
dcterms:title
Robust carbohydrate dynamics based on sucrose resynthesis in developing Norway spruce somatic embryos at variable sugar supply Robust carbohydrate dynamics based on sucrose resynthesis in developing Norway spruce somatic embryos at variable sugar supply
skos:prefLabel
Robust carbohydrate dynamics based on sucrose resynthesis in developing Norway spruce somatic embryos at variable sugar supply Robust carbohydrate dynamics based on sucrose resynthesis in developing Norway spruce somatic embryos at variable sugar supply
skos:notation
RIV/61389030:_____/14:00429973!RIV15-AV0-61389030
n5:aktivita
n6:I
n5:aktivity
I
n5:cisloPeriodika
1
n5:dodaniDat
n17:2015
n5:domaciTvurceVysledku
n14:7994427
n5:druhVysledku
n8:J
n5:duvernostUdaju
n15:S
n5:entitaPredkladatele
n10:predkladatel
n5:idSjednocenehoVysledku
42960
n5:idVysledku
RIV/61389030:_____/14:00429973
n5:jazykVysledku
n7:eng
n5:klicovaSlova
Sucrose; Raffinose family oligosaccharides; Picea abies
n5:klicoveSlovo
n11:Picea%20abies n11:Sucrose n11:Raffinose%20family%20oligosaccharides
n5:kodStatuVydavatele
US - Spojené státy americké
n5:kontrolniKodProRIV
[821DF1981CC0]
n5:nazevZdroje
In Vitro Celluar and Developmental Biology - Plant
n5:obor
n9:EF
n5:pocetDomacichTvurcuVysledku
1
n5:pocetTvurcuVysledku
4
n5:rokUplatneniVysledku
n17:2014
n5:svazekPeriodika
50
n5:tvurceVysledku
Kubeš, Martin Drážná, N. Konrádová, H. Lipavská, H.
n5:wos
000332456700006
s:issn
1054-5476
s:numberOfPages
13
n4:doi
10.1007/s11627-013-9589-6