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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F14%3A00429595%21RIV15-AV0-61389030
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Degradation of the plant hormone cytokinin is catalyzed by cytokinin oxidase/dehydrogenase (CKX) enzymes. The Arabidopsis thaliana genome encodes seven CKX proteins which differ in subcellular localization and substrate specificity. Here we analyze the CKX7 gene, which to the best of our knowledge has not yet been studied. pCKX7:GUS expression was detected in the vasculature, the transmitting tissue and the mature embryo sac. A CKX7-GFP fusion protein localized to the cytosol, which is unique among all CKX family members. 35S:CKX7-expressing plants developed short, early terminating primary roots with smaller apical meristems, contrasting with plants overexpressing other CKX genes. The vascular bundles of 35S:CKX7 primary roots contained only protoxylem elements, thus resembling the wol mutant of the CRE1/AHK4 receptor gene. We show that CRE1/AHK4 activity is required to establish the CKX7 overexpression phenotype. Several cytokinin metabolites, in particular cis-zeatin (cZ) and N-glucoside cytokinins, were depleted stronger in 35S:CKX7 plants compared with plants overexpressing other CKX genes. Interestingly, enhanced protoxylem formation together with reduced primary root growth was also found in the cZ-deficient tRNA isopentenyltransferase mutant ipt2,9. However, different cytokinins were similarly efficient in suppressing 35S:CKX7 and ipt2,9 vascular phenotypes. Therefore, we hypothesize that the pool of cytosolic cytokinins is particularly relevant in the root procambium where it mediates the differentiation of vascular tissues through CRE1/AHK4. Taken together, the distinct consequences of CKX7 overexpression indicate that the cellular compartmentalization of cytokinin degradation and substrate preference of CKX isoforms are relevant parameters that define the activities of the hormone. Degradation of the plant hormone cytokinin is catalyzed by cytokinin oxidase/dehydrogenase (CKX) enzymes. The Arabidopsis thaliana genome encodes seven CKX proteins which differ in subcellular localization and substrate specificity. Here we analyze the CKX7 gene, which to the best of our knowledge has not yet been studied. pCKX7:GUS expression was detected in the vasculature, the transmitting tissue and the mature embryo sac. A CKX7-GFP fusion protein localized to the cytosol, which is unique among all CKX family members. 35S:CKX7-expressing plants developed short, early terminating primary roots with smaller apical meristems, contrasting with plants overexpressing other CKX genes. The vascular bundles of 35S:CKX7 primary roots contained only protoxylem elements, thus resembling the wol mutant of the CRE1/AHK4 receptor gene. We show that CRE1/AHK4 activity is required to establish the CKX7 overexpression phenotype. Several cytokinin metabolites, in particular cis-zeatin (cZ) and N-glucoside cytokinins, were depleted stronger in 35S:CKX7 plants compared with plants overexpressing other CKX genes. Interestingly, enhanced protoxylem formation together with reduced primary root growth was also found in the cZ-deficient tRNA isopentenyltransferase mutant ipt2,9. However, different cytokinins were similarly efficient in suppressing 35S:CKX7 and ipt2,9 vascular phenotypes. Therefore, we hypothesize that the pool of cytosolic cytokinins is particularly relevant in the root procambium where it mediates the differentiation of vascular tissues through CRE1/AHK4. Taken together, the distinct consequences of CKX7 overexpression indicate that the cellular compartmentalization of cytokinin degradation and substrate preference of CKX isoforms are relevant parameters that define the activities of the hormone.
dcterms:title
Overexpression of the cytosolic cytokinin oxidase/dehydrogenase (CKX7) from Arabidopsis causes specific changes in root growth and xylem differentiation Overexpression of the cytosolic cytokinin oxidase/dehydrogenase (CKX7) from Arabidopsis causes specific changes in root growth and xylem differentiation
skos:prefLabel
Overexpression of the cytosolic cytokinin oxidase/dehydrogenase (CKX7) from Arabidopsis causes specific changes in root growth and xylem differentiation Overexpression of the cytosolic cytokinin oxidase/dehydrogenase (CKX7) from Arabidopsis causes specific changes in root growth and xylem differentiation
skos:notation
RIV/61389030:_____/14:00429595!RIV15-AV0-61389030
n4:aktivita
n17:S n17:I
n4:aktivity
I, S
n4:cisloPeriodika
3
n4:dodaniDat
n6:2015
n4:domaciTvurceVysledku
n13:3686957 n13:4712048
n4:druhVysledku
n15:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
35479
n4:idVysledku
RIV/61389030:_____/14:00429595
n4:jazykVysledku
n8:eng
n4:klicovaSlova
xylem differentiation; Arabidopsis thaliana; cytokinin oxidase/dehydrogenase
n4:klicoveSlovo
n11:cytokinin%20oxidase%2Fdehydrogenase n11:xylem%20differentiation n11:Arabidopsis%20thaliana
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[9684B7B4CC25]
n4:nazevZdroje
Plant Journal
n4:obor
n16:ED
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
5
n4:rokUplatneniVysledku
n6:2014
n4:svazekPeriodika
78
n4:tvurceVysledku
Novák, Ondřej Strnad, Miroslav Kollmer, I. Schmülling, T. Werner, T.
n4:wos
000334824300001
s:issn
0960-7412
s:numberOfPages
13
n12:doi
10.1111/tpj.12477