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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F13%3A00440716%21RIV15-GA0-61389030
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Male gametogenesis in flowering plants is attributed by substantial changes in cellular reorganization, cell fate specification and changes in cellular metabolism including RNA metabolism. Sequestration from immediate translation of protein coding mRNAs has emerged as one aspect of mRNA post transcriptional regulation that is widespread during gametogenesis. Transcripts repressed from translation are confiscated into mRNA storing bodies and function later during development and at early stages of embryogenesis. In Arabidopsis, 88% and 12% of the zygotic transcriptome at 2-4 cells stage of development now known to originate from maternal and paternal gametes respectively. Moreover, an activator of YODA (YDA) mitogen-activated protein kinase pathway in the zygote, the interleukin-1 receptor-associated kinase (IRAK)/Pelle-like kinase gene Short Suspensor (SSP) is specifically expressed in the Arabidopsis male gametophyte but only translated upon gamete fusion to promote suspensor development. Previously we have reported the proteome of EPP complexes, the tobacco pollen large mRNPs granules containing translationally sequestered mRNAs, non-coding smRNAs and a repertoire of associated protein complexes including factors for initiation of translation. Here, we have analysed our previous and new EPP proteome data set obtained from orbitrap LC-MS/MS as well as tobacco pollen Agilent microarray data and highlighted that RNA helicases, predominantly DEAD-box domain containing helicases are among the constituents of the EPP RNA granules. Their potential function tied to local translation is explored. In addition, we discuss the recent report of RNA helicases localization within peroxisomes bodies and speculate their likely role as an additional site for mRNA control in the male gametophyte alongside EPP mRNA storing granules. Thus, mRNA homeostasis in the male gametophyte might be subjected to multiple controls at diverse but likely interconnected cytoplasmic foci. Male gametogenesis in flowering plants is attributed by substantial changes in cellular reorganization, cell fate specification and changes in cellular metabolism including RNA metabolism. Sequestration from immediate translation of protein coding mRNAs has emerged as one aspect of mRNA post transcriptional regulation that is widespread during gametogenesis. Transcripts repressed from translation are confiscated into mRNA storing bodies and function later during development and at early stages of embryogenesis. In Arabidopsis, 88% and 12% of the zygotic transcriptome at 2-4 cells stage of development now known to originate from maternal and paternal gametes respectively. Moreover, an activator of YODA (YDA) mitogen-activated protein kinase pathway in the zygote, the interleukin-1 receptor-associated kinase (IRAK)/Pelle-like kinase gene Short Suspensor (SSP) is specifically expressed in the Arabidopsis male gametophyte but only translated upon gamete fusion to promote suspensor development. Previously we have reported the proteome of EPP complexes, the tobacco pollen large mRNPs granules containing translationally sequestered mRNAs, non-coding smRNAs and a repertoire of associated protein complexes including factors for initiation of translation. Here, we have analysed our previous and new EPP proteome data set obtained from orbitrap LC-MS/MS as well as tobacco pollen Agilent microarray data and highlighted that RNA helicases, predominantly DEAD-box domain containing helicases are among the constituents of the EPP RNA granules. Their potential function tied to local translation is explored. In addition, we discuss the recent report of RNA helicases localization within peroxisomes bodies and speculate their likely role as an additional site for mRNA control in the male gametophyte alongside EPP mRNA storing granules. Thus, mRNA homeostasis in the male gametophyte might be subjected to multiple controls at diverse but likely interconnected cytoplasmic foci.
dcterms:title
DEAD-Box RNA Helicases are among the Constituents of the Tobacco Pollen mRNA Storing Bodies DEAD-Box RNA Helicases are among the Constituents of the Tobacco Pollen mRNA Storing Bodies
skos:prefLabel
DEAD-Box RNA Helicases are among the Constituents of the Tobacco Pollen mRNA Storing Bodies DEAD-Box RNA Helicases are among the Constituents of the Tobacco Pollen mRNA Storing Bodies
skos:notation
RIV/61389030:_____/13:00440716!RIV15-GA0-61389030
n3:aktivita
n5:P n5:I
n3:aktivity
I, P(GAP501/11/1462), P(GPP501/11/P321), P(LD13049)
n3:cisloPeriodika
3
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n10:3997073 n10:5468582
n3:druhVysledku
n9:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
68052
n3:idVysledku
RIV/61389030:_____/13:00440716
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Translation; mRNA storage; RNA helicase
n3:klicoveSlovo
n14:RNA%20helicase n14:Translation n14:mRNA%20storage
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[FE359FD30C39]
n3:nazevZdroje
Journal of Plant Biochemistry and Physiology
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n4:LD13049 n4:GPP501%2F11%2FP321 n4:GAP501%2F11%2F1462
n3:rokUplatneniVysledku
n11:2013
n3:svazekPeriodika
1
n3:tvurceVysledku
Potěšil, D. Honys, David Hafidh, Said Zdráhal, Z.
s:issn
2329-9029
s:numberOfPages
4
n7:doi
10.4172/2329-9029.1000114