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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F13%3A00399598%21RIV14-AV0-61389030
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Cytokinins (CK) play an important role in the formation of nitrogen-fixing root nodules. It has been known for years that rhizobia secrete CK in the extracellular medium but whether they play a role in nodule formation is not known. We have examined this question using the photosynthetic Bradyrhizobium sp. strain ORS285 which is able to nodulate Aeschynomene afraspera and A. indica using a Nod-dependent or Nod-independent symbiotic process, respectively. CK profiling showed that the most abundant CK secreted by Bradyrhizobium sp. strain ORS285 are the 2MeS (2-methylthiol) derivatives of trans-zeatin and isopentenyladenine. In their pure form, these CK can activate legume CK receptors in vitro, and their exogenous addition induced nodule-like structures on host plants. Deletion of the miaA gene showed that transfer RNA degradation is the source of CK production in Bradyrhizobium sp. strain ORS285. In nodulation studies performed with A. indica and A. afraspera, the miaA mutant had a 1-day delay in nodulation and nitrogen fixation. Moreover, A. indica plants formed considerably smaller but more abundant nodules when inoculated with the miaA mutant. These data show that CK produced by Bradyrhizobium sp. strain ORS285 are not the key signal triggering nodule formation during the Nod-independent symbiosis but they contribute positively to nodule development in Aeschynomene plants. Cytokinins (CK) play an important role in the formation of nitrogen-fixing root nodules. It has been known for years that rhizobia secrete CK in the extracellular medium but whether they play a role in nodule formation is not known. We have examined this question using the photosynthetic Bradyrhizobium sp. strain ORS285 which is able to nodulate Aeschynomene afraspera and A. indica using a Nod-dependent or Nod-independent symbiotic process, respectively. CK profiling showed that the most abundant CK secreted by Bradyrhizobium sp. strain ORS285 are the 2MeS (2-methylthiol) derivatives of trans-zeatin and isopentenyladenine. In their pure form, these CK can activate legume CK receptors in vitro, and their exogenous addition induced nodule-like structures on host plants. Deletion of the miaA gene showed that transfer RNA degradation is the source of CK production in Bradyrhizobium sp. strain ORS285. In nodulation studies performed with A. indica and A. afraspera, the miaA mutant had a 1-day delay in nodulation and nitrogen fixation. Moreover, A. indica plants formed considerably smaller but more abundant nodules when inoculated with the miaA mutant. These data show that CK produced by Bradyrhizobium sp. strain ORS285 are not the key signal triggering nodule formation during the Nod-independent symbiosis but they contribute positively to nodule development in Aeschynomene plants.
dcterms:title
Rhizobial Synthesized Cytokinins Contribute to But Are Not Essential for the Symbiotic Interaction Between Photosynthetic Bradyrhizobia and Aeschynomene Legumes Rhizobial Synthesized Cytokinins Contribute to But Are Not Essential for the Symbiotic Interaction Between Photosynthetic Bradyrhizobia and Aeschynomene Legumes
skos:prefLabel
Rhizobial Synthesized Cytokinins Contribute to But Are Not Essential for the Symbiotic Interaction Between Photosynthetic Bradyrhizobia and Aeschynomene Legumes Rhizobial Synthesized Cytokinins Contribute to But Are Not Essential for the Symbiotic Interaction Between Photosynthetic Bradyrhizobia and Aeschynomene Legumes
skos:notation
RIV/61389030:_____/13:00399598!RIV14-AV0-61389030
n14:predkladatel
n15:ico%3A61389030
n3:aktivita
n8:P n8:Z
n3:aktivity
P(ED0007/01/01), Z(AV0Z50380511)
n3:cisloPeriodika
10
n3:dodaniDat
n17:2014
n3:domaciTvurceVysledku
n6:4712048 n6:6422764 n6:3686957 n6:1749560
n3:druhVysledku
n20:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
102905
n3:idVysledku
RIV/61389030:_____/13:00399598
n3:jazykVysledku
n10:eng
n3:klicovaSlova
TANDEM MASS-SPECTROMETRY; LOTUS-JAPONICUS; NODULE ORGANOGENESIS
n3:klicoveSlovo
n4:TANDEM%20MASS-SPECTROMETRY n4:LOTUS-JAPONICUS n4:NODULE%20ORGANOGENESIS
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[B067CAB4B6EB]
n3:nazevZdroje
Molecular Plant-Microbe Interactions
n3:obor
n16:EF
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
9
n3:projekt
n7:ED0007%2F01%2F01
n3:rokUplatneniVysledku
n17:2013
n3:svazekPeriodika
26
n3:tvurceVysledku
Giraud, E. Bonaldi, K. Novák, Ondřej Podlešáková, Kateřina Patrel, D. Spíchal, Lukáš Fardoux, J. Nouwen, N. Strnad, Miroslav
n3:wos
000324347800012
n3:zamer
n11:AV0Z50380511
s:issn
0894-0282
s:numberOfPages
7
n13:doi
10.1094/MPMI-03-13-0076-R