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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F13%3A00396875%21RIV14-GA0-61389030
rdf:type
skos:Concept n11:Vysledek
dcterms:description
In this study, data is presented how dark-grown, embryogenic barley callus cells respond to cold without any light-dependent, chloroplast-related mechanism, independently of the systemic signals. The expression of HvCBF9, HvCBF14, and HvCOR14b genes, members of one of the most important cold-inducible regulatory system, was measured by real-time PCR. Characteristic of the cold response was similar in the crowns of seedlings and in dark-grown callus cultures, however, gene expression levels were lower in calli. Endogenous concentration of auxins, abscisic acid, and salicylic acid did not change, but phaseic acid and neophaseic acid showed robust accumulation after cold acclimation. Freezing tolerance of the cultures was also higher after 7 days of cold-hardening. The results suggest the presence of a basal, light-independent, cold-responsive activation of the CBF-COR14b pathway in barley cultures. The effects of Dicamba, the exogenous auxin analog used for maintaining tissue cultures were also studied. Dicamba seems to be a general enhancer of the gene expression and physiological responses to cold stress, but has no specific effect on the activation. Our data along with previous findings show that this system might be a suitable model for studying certain basic cellular mechanisms involved in the cold acclimation process in cereals. In this study, data is presented how dark-grown, embryogenic barley callus cells respond to cold without any light-dependent, chloroplast-related mechanism, independently of the systemic signals. The expression of HvCBF9, HvCBF14, and HvCOR14b genes, members of one of the most important cold-inducible regulatory system, was measured by real-time PCR. Characteristic of the cold response was similar in the crowns of seedlings and in dark-grown callus cultures, however, gene expression levels were lower in calli. Endogenous concentration of auxins, abscisic acid, and salicylic acid did not change, but phaseic acid and neophaseic acid showed robust accumulation after cold acclimation. Freezing tolerance of the cultures was also higher after 7 days of cold-hardening. The results suggest the presence of a basal, light-independent, cold-responsive activation of the CBF-COR14b pathway in barley cultures. The effects of Dicamba, the exogenous auxin analog used for maintaining tissue cultures were also studied. Dicamba seems to be a general enhancer of the gene expression and physiological responses to cold stress, but has no specific effect on the activation. Our data along with previous findings show that this system might be a suitable model for studying certain basic cellular mechanisms involved in the cold acclimation process in cereals.
dcterms:title
Cold Response of Dedifferentiated Barley Cells at the Gene Expression, Hormone Composition, and Freezing Tolerance Levels: Studies on Callus Cultures Cold Response of Dedifferentiated Barley Cells at the Gene Expression, Hormone Composition, and Freezing Tolerance Levels: Studies on Callus Cultures
skos:prefLabel
Cold Response of Dedifferentiated Barley Cells at the Gene Expression, Hormone Composition, and Freezing Tolerance Levels: Studies on Callus Cultures Cold Response of Dedifferentiated Barley Cells at the Gene Expression, Hormone Composition, and Freezing Tolerance Levels: Studies on Callus Cultures
skos:notation
RIV/61389030:_____/13:00396875!RIV14-GA0-61389030
n11:predkladatel
n12:ico%3A61389030
n4:aktivita
n5:P n5:Z
n4:aktivity
P(GA522/09/2058), Z(AV0Z50380511)
n4:cisloPeriodika
2
n4:dodaniDat
n15:2014
n4:domaciTvurceVysledku
n16:9744886 n16:4739841
n4:druhVysledku
n9:J
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n7:predkladatel
n4:idSjednocenehoVysledku
65899
n4:idVysledku
RIV/61389030:_____/13:00396875
n4:jazykVysledku
n8:eng
n4:klicovaSlova
ABA; Barley; Callus
n4:klicoveSlovo
n13:Barley n13:Callus n13:ABA
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[52D49AB0EB13]
n4:nazevZdroje
Molecular Biotechnology
n4:obor
n14:ED
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
6
n4:projekt
n20:GA522%2F09%2F2058
n4:rokUplatneniVysledku
n15:2013
n4:svazekPeriodika
54
n4:tvurceVysledku
Marozsan-Toth, Z. Vashegyi, I. Dobrev, Petre Galiba, G. Vaňková, Radomíra Toth, B.
n4:wos
000318308400025
n4:zamer
n10:AV0Z50380511
s:issn
1073-6085
s:numberOfPages
13
n18:doi
10.1007/s12033-012-9569-9