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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F12%3A00390495%21RIV13-AV0-61389030
rdf:type
skos:Concept n16:Vysledek
rdfs:seeAlso
http://www.intechopen.com/books/howtoreference/embryogenesis/role-of-polyamines-in-efficiency-of-norway-spruce-hurst-ecotype-somatic-embryogenesis-
dcterms:description
Micropropagation technologies represent a powerful tool for improvement and acceleration of tree breeding programs in forestry. Tissue culture approaches, in particular somatic embryogenesis, hold considerable promise for breeding programs of coniferous trees. Utilization of somatic embryogenesis could facilitate reproduction of rare or selected coniferous genotypes. Despite the availability of a successful protocol for generating Norway spruce plants using a somatic embryogenesis technique, there is a lack of data concerning the endogenous composition of biologically active compounds in somatic embryos. Beside the key roles of auxins and cytokinins, very important function in differentiation processes belongs to polyamines although the mechanism of their action is still not fully cleared. Positive correlation between embryogenic capacity and total content of free PAs confirmed crucial role of PAs during somatic embryo development. It was shown that the predominant PAs in somatic embryos of highly responsible Hurst ecotype of Norway spruce (L10) was spermidine, while embryos of less responsible ecotype (L13, L16, L17 and L28) contained lower level of Spd and/or inadequate Put/Spd ratio. Exogenously supplied PAs might therefore affect and improve the induction and somatic embryo development in less responsible plant genotypes. The studies related to the improving of somatic embryogenesis method in less responsible Hurst ecotype of Norway spruce are now in progress in our laboratory. Micropropagation technologies represent a powerful tool for improvement and acceleration of tree breeding programs in forestry. Tissue culture approaches, in particular somatic embryogenesis, hold considerable promise for breeding programs of coniferous trees. Utilization of somatic embryogenesis could facilitate reproduction of rare or selected coniferous genotypes. Despite the availability of a successful protocol for generating Norway spruce plants using a somatic embryogenesis technique, there is a lack of data concerning the endogenous composition of biologically active compounds in somatic embryos. Beside the key roles of auxins and cytokinins, very important function in differentiation processes belongs to polyamines although the mechanism of their action is still not fully cleared. Positive correlation between embryogenic capacity and total content of free PAs confirmed crucial role of PAs during somatic embryo development. It was shown that the predominant PAs in somatic embryos of highly responsible Hurst ecotype of Norway spruce (L10) was spermidine, while embryos of less responsible ecotype (L13, L16, L17 and L28) contained lower level of Spd and/or inadequate Put/Spd ratio. Exogenously supplied PAs might therefore affect and improve the induction and somatic embryo development in less responsible plant genotypes. The studies related to the improving of somatic embryogenesis method in less responsible Hurst ecotype of Norway spruce are now in progress in our laboratory.
dcterms:title
Role of Polyamines in Efficiency of Norway Spruce (Hurst Ecotype) Somatic Embryogenesis Role of Polyamines in Efficiency of Norway Spruce (Hurst Ecotype) Somatic Embryogenesis
skos:prefLabel
Role of Polyamines in Efficiency of Norway Spruce (Hurst Ecotype) Somatic Embryogenesis Role of Polyamines in Efficiency of Norway Spruce (Hurst Ecotype) Somatic Embryogenesis
skos:notation
RIV/61389030:_____/12:00390495!RIV13-AV0-61389030
n16:predkladatel
n17:ico%3A61389030
n3:aktivita
n12:Z n12:P
n3:aktivity
P(QH82303), Z(AV0Z50380511)
n3:dodaniDat
n13:2013
n3:domaciTvurceVysledku
n11:1304372 n11:6225578
n3:druhVysledku
n19:C
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
166044
n3:idVysledku
RIV/61389030:_____/12:00390495
n3:jazykVysledku
n22:eng
n3:klicovaSlova
Norway Spruce; Somatic Embryogenesis; Polyamines
n3:klicoveSlovo
n8:Norway%20Spruce n8:Polyamines n8:Somatic%20Embryogenesis
n3:kontrolniKodProRIV
[402A8C973370]
n3:mistoVydani
Rijeka
n3:nazevZdroje
Embryogenesis
n3:obor
n18:GK
n3:pocetDomacichTvurcuVysledku
2
n3:pocetStranKnihy
652
n3:pocetTvurcuVysledku
4
n3:projekt
n23:QH82303
n3:rokUplatneniVysledku
n13:2012
n3:tvurceVysledku
Gemperlová, Lenka Máchová, P. Malá, J. Cvikrová, Milena
n3:zamer
n5:AV0Z50380511
s:numberOfPages
14
n21:doi
10.5772/2143
n10:hasPublisher
InTech
n15:isbn
978-953-51-0466-7