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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F12%3A00381390%21RIV13-AV0-61389030
rdf:type
n14:Vysledek skos:Concept
dcterms:description
In this study, a survey of miRNAs using the next-generation sequencing data was performed at subgenomic level. After analyzing shotgun sequences from chromosome 4A of bread wheat (Triticum aestivum L.), a total of 68 different miRNAs were predicted in silico, of which 37 were identified in wheat for the first time. The long arm of the chromosome was found to harbor a higher variety (51) and representation (3,928) of miRNAs compared with the short arm (49; 2,226). Out of the 68 miRNAs, 32 were detected to be common to both arms, revealing the presence of separate miRNA clusters in the two chromosome arms. The differences in degree of representation of the different miRNAs were found to be highly variable, ranging 592-fold, which may have an effect on target regulation. Targets were retrieved for 62 (out of 68) of wheat-specific, newly identified miRNAs indicated that fundamental aspects of plant morphology such as height and flowering were predicted to be affected. In silico expression blast analysis indicated 24 (out of 68) were found to give hits to expressed sequences. This is the first report of species- and chromosome-specific miRNAs. In this study, a survey of miRNAs using the next-generation sequencing data was performed at subgenomic level. After analyzing shotgun sequences from chromosome 4A of bread wheat (Triticum aestivum L.), a total of 68 different miRNAs were predicted in silico, of which 37 were identified in wheat for the first time. The long arm of the chromosome was found to harbor a higher variety (51) and representation (3,928) of miRNAs compared with the short arm (49; 2,226). Out of the 68 miRNAs, 32 were detected to be common to both arms, revealing the presence of separate miRNA clusters in the two chromosome arms. The differences in degree of representation of the different miRNAs were found to be highly variable, ranging 592-fold, which may have an effect on target regulation. Targets were retrieved for 62 (out of 68) of wheat-specific, newly identified miRNAs indicated that fundamental aspects of plant morphology such as height and flowering were predicted to be affected. In silico expression blast analysis indicated 24 (out of 68) were found to give hits to expressed sequences. This is the first report of species- and chromosome-specific miRNAs.
dcterms:title
Subgenomic analysis of microRNAs in polyploid wheat Subgenomic analysis of microRNAs in polyploid wheat
skos:prefLabel
Subgenomic analysis of microRNAs in polyploid wheat Subgenomic analysis of microRNAs in polyploid wheat
skos:notation
RIV/61389030:_____/12:00381390!RIV13-AV0-61389030
n14:predkladatel
n15:ico%3A61389030
n4:aktivita
n8:Z
n4:aktivity
Z(AV0Z50380511)
n4:cisloPeriodika
3
n4:dodaniDat
n5:2013
n4:domaciTvurceVysledku
n10:5373638 n10:1800477
n4:druhVysledku
n13:J
n4:duvernostUdaju
n7:S
n4:entitaPredkladatele
n19:predkladatel
n4:idSjednocenehoVysledku
172296
n4:idVysledku
RIV/61389030:_____/12:00381390
n4:jazykVysledku
n18:eng
n4:klicovaSlova
Triticum aestivum; microRNA; miRNA prediction
n4:klicoveSlovo
n6:Triticum%20aestivum n6:miRNA%20prediction n6:microRNA
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[6343426C4C69]
n4:nazevZdroje
FUNCTIONAL & INTEGRATIVE GENOMICS
n4:obor
n16:EB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
7
n4:rokUplatneniVysledku
n5:2012
n4:svazekPeriodika
12
n4:tvurceVysledku
Lucas, S. J. Valárik, Miroslav Hernandez, P. Kantar, M. Doležel, Jaroslav Budak, H. Akpinar, B. A.
n4:wos
000308238900005
n4:zamer
n12:AV0Z50380511
s:issn
1438-793X
s:numberOfPages
15
n17:doi
10.1007/s10142-012-0285-0