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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F12%3A00381153%21RIV13-AV0-61389030
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing. Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing.
dcterms:title
Flow cytometric chromosome sorting in plants: The next generation Flow cytometric chromosome sorting in plants: The next generation
skos:prefLabel
Flow cytometric chromosome sorting in plants: The next generation Flow cytometric chromosome sorting in plants: The next generation
skos:notation
RIV/61389030:_____/12:00381153!RIV13-AV0-61389030
n15:predkladatel
n19:ico%3A61389030
n3:aktivita
n10:P n10:Z
n3:aktivity
P(GAP501/10/1740), Z(AV0Z50380511)
n3:cisloPeriodika
3
n3:dodaniDat
n13:2013
n3:domaciTvurceVysledku
n5:1800477 n5:9606599 n5:9544887 n5:5207010 n5:5256593
n3:druhVysledku
n20:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
136827
n3:idVysledku
RIV/61389030:_____/12:00381153
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Chromosome sorting; Flow cytometry; Fluorescence in situ hybridization
n3:klicoveSlovo
n11:Chromosome%20sorting n11:Fluorescence%20in%20situ%20hybridization n11:Flow%20cytometry
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[2DA843F49310]
n3:nazevZdroje
Methods
n3:obor
n6:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n4:GAP501%2F10%2F1740
n3:rokUplatneniVysledku
n13:2012
n3:svazekPeriodika
57
n3:tvurceVysledku
Doležel, Jaroslav Číhalíková, Jarmila Kubaláková, Marie Vrána, Jan Šimková, Hana
n3:wos
000308852400009
n3:zamer
n8:AV0Z50380511
s:issn
1046-2023
s:numberOfPages
7
n17:doi
10.1016/j.ymeth.2012.03.006