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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F12%3A00381055%21RIV13-AV0-61389030
rdf:type
n6:Vysledek skos:Concept
dcterms:description
The Potato virus X (PVX)-based vector was used for the construction of N- and C-terminally modified PVX coat protein (XCP) chimeras. N-terminal XCP modifications do not influence the viral life cycle, whereas the simple XCP C-terminal fusion impedes the viral replication. We designed several C-terminally modified XCP chimeras and tested their viabilities in various Nicotiana benthamiana genotypes. Our results showed the negative impact of 3'-terminal modification of XCP on the chimera's life cycle. To ensure chimeric constructs stability, the second copy of the last 60 nucleotides of XCP followed by the 3'-untranslated region (UTR) was added downstream of the recombinant sequence. Simultaneously, the first copy of the last 60 nucleotides of XCP was mutated in order to prevent recombination between the two identical sequences. The movement protein of Tobacco mosaic virus expressed in transgenic N. benthamiana plants positively affected the cell-to-cell spread of C-terminally modified XCP chimeras. The Potato virus X (PVX)-based vector was used for the construction of N- and C-terminally modified PVX coat protein (XCP) chimeras. N-terminal XCP modifications do not influence the viral life cycle, whereas the simple XCP C-terminal fusion impedes the viral replication. We designed several C-terminally modified XCP chimeras and tested their viabilities in various Nicotiana benthamiana genotypes. Our results showed the negative impact of 3'-terminal modification of XCP on the chimera's life cycle. To ensure chimeric constructs stability, the second copy of the last 60 nucleotides of XCP followed by the 3'-untranslated region (UTR) was added downstream of the recombinant sequence. Simultaneously, the first copy of the last 60 nucleotides of XCP was mutated in order to prevent recombination between the two identical sequences. The movement protein of Tobacco mosaic virus expressed in transgenic N. benthamiana plants positively affected the cell-to-cell spread of C-terminally modified XCP chimeras.
dcterms:title
The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties
skos:prefLabel
The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties
skos:notation
RIV/61389030:_____/12:00381055!RIV13-AV0-61389030
n6:predkladatel
n11:ico%3A61389030
n3:aktivita
n8:Z n8:S n8:P n8:I
n3:aktivity
I, P(GA521/09/1525), S, Z(AV0Z50380511)
n3:cisloPeriodika
4
n3:dodaniDat
n10:2013
n3:domaciTvurceVysledku
n5:7629923 n5:7888848 n5:9833242 n5:8618348 n5:3229475
n3:druhVysledku
n15:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
141633
n3:idVysledku
RIV/61389030:_____/12:00381055
n3:jazykVysledku
n13:eng
n3:klicovaSlova
chimeric coat protein; expression of recombinant protein; Nicotiana benthamiana
n3:klicoveSlovo
n9:Nicotiana%20benthamiana n9:chimeric%20coat%20protein n9:expression%20of%20recombinant%20protein
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[2508F1212D89]
n3:nazevZdroje
Biologia Plantarum
n3:obor
n18:EI
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n14:GA521%2F09%2F1525
n3:rokUplatneniVysledku
n10:2012
n3:svazekPeriodika
56
n3:tvurceVysledku
Čeřovská, Noemi Plchová, Helena Moravec, Tomáš Folwarczna, Jitka Hoffmeisterová, Hana
n3:wos
000305602100027
n3:zamer
n17:AV0Z50380511
s:issn
0006-3134
s:numberOfPages
5
n20:doi
10.1007/s10535-012-0130-2