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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F12%3A00380702%21RIV13-AV0-61389030
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Cross-talk between plant cells and their surroundings requires tight regulation of information exchange at the plasma membrane (PM), which involves dynamic adjustments of PM protein localization and turnover to modulate signal perception and solute transport at the interface between cells and their surroundings. In animals and fungi, turnover of PM proteins is controlled by reversible ubiquitylation, which signals endocytosis and delivery to the cell’s lytic compartment, and there is emerging evidence for related mechanisms in plants. Here, we describe the fate of Arabidopsis PIN2 protein, required for directional cellular efflux of the phytohormone auxin, and identify cis- and trans-acting mediators of PIN2 ubiquitylation. We demonstrate that ubiquitin acts as a principal signal for PM protein endocytosis in plants and reveal dynamic adjustments in PIN2 ubiquitylation coinciding with variations in vacuolar targeting and proteolytic turnover. We show that control of PIN2 proteolytic turnover via its ubiquitylation status is of significant importance for auxin distribution in root meristems and for environmentally controlled adaptations of root growth. Moreover, we provide experimental evidence indicating that PIN2 vacuolar sorting depends on modification specifically by lysine63-linked ubiquitin chains. Collectively, our results establish lysine63-linked PM cargo ubiquitylation as a regulator of polar auxin transport and adaptive growth responses in higher plants. Cross-talk between plant cells and their surroundings requires tight regulation of information exchange at the plasma membrane (PM), which involves dynamic adjustments of PM protein localization and turnover to modulate signal perception and solute transport at the interface between cells and their surroundings. In animals and fungi, turnover of PM proteins is controlled by reversible ubiquitylation, which signals endocytosis and delivery to the cell’s lytic compartment, and there is emerging evidence for related mechanisms in plants. Here, we describe the fate of Arabidopsis PIN2 protein, required for directional cellular efflux of the phytohormone auxin, and identify cis- and trans-acting mediators of PIN2 ubiquitylation. We demonstrate that ubiquitin acts as a principal signal for PM protein endocytosis in plants and reveal dynamic adjustments in PIN2 ubiquitylation coinciding with variations in vacuolar targeting and proteolytic turnover. We show that control of PIN2 proteolytic turnover via its ubiquitylation status is of significant importance for auxin distribution in root meristems and for environmentally controlled adaptations of root growth. Moreover, we provide experimental evidence indicating that PIN2 vacuolar sorting depends on modification specifically by lysine63-linked ubiquitin chains. Collectively, our results establish lysine63-linked PM cargo ubiquitylation as a regulator of polar auxin transport and adaptive growth responses in higher plants.
dcterms:title
Lysine(63)-linked ubiquitylation of PIN2 auxin carrier protein governs hormonally controlled adaptation of Arabidopsis root growth Lysine(63)-linked ubiquitylation of PIN2 auxin carrier protein governs hormonally controlled adaptation of Arabidopsis root growth
skos:prefLabel
Lysine(63)-linked ubiquitylation of PIN2 auxin carrier protein governs hormonally controlled adaptation of Arabidopsis root growth Lysine(63)-linked ubiquitylation of PIN2 auxin carrier protein governs hormonally controlled adaptation of Arabidopsis root growth
skos:notation
RIV/61389030:_____/12:00380702!RIV13-AV0-61389030
n8:predkladatel
n17:ico%3A61389030
n3:aktivita
n16:P n16:Z
n3:aktivity
P(GAP305/11/2476), Z(AV0Z50380511)
n3:cisloPeriodika
21
n3:dodaniDat
n10:2013
n3:domaciTvurceVysledku
n14:3610659 n14:2027178 n14:5408954
n3:druhVysledku
n11:J
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
147628
n3:idVysledku
RIV/61389030:_____/12:00380702
n3:jazykVysledku
n12:eng
n3:klicovaSlova
PLASMA-MEMBRANE PROTEIN; EFFLUX CARRIER; INTRACELLULAR TRAFFICKING
n3:klicoveSlovo
n15:PLASMA-MEMBRANE%20PROTEIN n15:INTRACELLULAR%20TRAFFICKING n15:EFFLUX%20CARRIER
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[7A638CEAAE6D]
n3:nazevZdroje
Proceedings of the National Academy of Sciences of the United States of America
n3:obor
n6:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
9
n3:projekt
n4:GAP305%2F11%2F2476
n3:rokUplatneniVysledku
n10:2012
n3:svazekPeriodika
109
n3:tvurceVysledku
Korbei, B. Zažímalová, Eva Pařezová, Markéta Retzer, K. Tomanov, K. Leitner, J. Bachmair, A. Luschnig, Ch. Petrášek, Jan
n3:wos
000304445800080
n3:zamer
n9:AV0Z50380511
s:issn
0027-8424
s:numberOfPages
6
n13:doi
10.1073/pnas.1200824109