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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F12%3A00366394%21RIV13-AV0-61389030
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Rapid changes of protein phosphorylation play a crucial role in the regulation of many cellular processes. Being post-translationally modified, phosphoproteins are often present in quite low abundance and tend to co-exist with their unphosphorylated isoform within the cell. To make their identification more practicable, the use of enrichment protocols is often required. The enrichment strategies can be performed either at the level of phosphoproteins or at the level of phosphopeptides. Both approaches have their advantages and disadvantages. Most enriching strategies are based on chemical modifications, affinity chromatography to capture peptides and proteins containing negatively charged phosphate groups onto a positively charged matrix, or immunoprecipitation by phospho-specific antibodies. In this article, the most up-to-date enrichment techniques are discussed, taking into account their optimization, and highlighting their advantages and disadvantages. Rapid changes of protein phosphorylation play a crucial role in the regulation of many cellular processes. Being post-translationally modified, phosphoproteins are often present in quite low abundance and tend to co-exist with their unphosphorylated isoform within the cell. To make their identification more practicable, the use of enrichment protocols is often required. The enrichment strategies can be performed either at the level of phosphoproteins or at the level of phosphopeptides. Both approaches have their advantages and disadvantages. Most enriching strategies are based on chemical modifications, affinity chromatography to capture peptides and proteins containing negatively charged phosphate groups onto a positively charged matrix, or immunoprecipitation by phospho-specific antibodies. In this article, the most up-to-date enrichment techniques are discussed, taking into account their optimization, and highlighting their advantages and disadvantages.
dcterms:title
Enrichment techniques employed in phosphoproteomics Enrichment techniques employed in phosphoproteomics
skos:prefLabel
Enrichment techniques employed in phosphoproteomics Enrichment techniques employed in phosphoproteomics
skos:notation
RIV/61389030:_____/12:00366394!RIV13-AV0-61389030
n12:predkladatel
n13:ico%3A61389030
n4:aktivita
n18:P n18:Z
n4:aktivity
P(GA522/09/0858), P(GA525/09/0994), P(GAP501/11/1462), P(OC08011), Z(AV0Z50380511)
n4:cisloPeriodika
3
n4:dodaniDat
n17:2013
n4:domaciTvurceVysledku
n15:5468582 n15:8943494
n4:druhVysledku
n16:J
n4:duvernostUdaju
n5:S
n4:entitaPredkladatele
n19:predkladatel
n4:idSjednocenehoVysledku
134462
n4:idVysledku
RIV/61389030:_____/12:00366394
n4:jazykVysledku
n11:eng
n4:klicovaSlova
Phosphoproteomics; Enrichment; IMAC
n4:klicoveSlovo
n20:Enrichment n20:Phosphoproteomics n20:IMAC
n4:kodStatuVydavatele
AT - Rakouská republika
n4:kontrolniKodProRIV
[4F7AD194C763]
n4:nazevZdroje
Amino Acids
n4:obor
n7:ED
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
2
n4:projekt
n10:GA522%2F09%2F0858 n10:OC08011 n10:GA525%2F09%2F0994 n10:GAP501%2F11%2F1462
n4:rokUplatneniVysledku
n17:2012
n4:svazekPeriodika
43
n4:tvurceVysledku
Honys, David Fíla, Jan
n4:wos
000307536100003
n4:zamer
n14:AV0Z50380511
s:issn
0939-4451
s:numberOfPages
23
n8:doi
10.1007/s00726-011-1111-z