This HTML5 document contains 49 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n13http://purl.org/net/nknouf/ns/bibtex#
n7http://linked.opendata.cz/resource/domain/vavai/projekt/
n4http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n18http://linked.opendata.cz/resource/domain/vavai/subjekt/
n17http://linked.opendata.cz/ontology/domain/vavai/
n20https://schema.org/
n15http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n16http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n11http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n22http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n12http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n19http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F61389030%3A_____%2F11%3A00375427%21RIV12-AV0-61389030/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n10http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F11%3A00375427%21RIV12-AV0-61389030
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Chromosome analysis and sorting using flow cytometry (flow cytogenetics) is an attractive tool for fractionating plant genomes to small parts. The reduction of complexity greatly simplifies genetics and genomics in plant species with large genomes. However, as flow cytometry requires liquid suspensions of particles, the lack of suitable protocols for preparation of solutions of intact chromosomes delayed the application of flow cytogenetics in plants. This chapter outlines a high-yielding procedure for preparation of solutions of intact mitotic chromosomes from root tips of young seedlings, their analysis using flow cytometry and sorting. Seedlings are easy to handle, root meristems are karyological stable, and high degree of metaphase synchrony can be induced. Chromosomes isolated according to this protocol have well preserved morphology, withstand shearing forces during sorting and their DNA is intact and suitable for a range of applications. Chromosome analysis and sorting using flow cytometry (flow cytogenetics) is an attractive tool for fractionating plant genomes to small parts. The reduction of complexity greatly simplifies genetics and genomics in plant species with large genomes. However, as flow cytometry requires liquid suspensions of particles, the lack of suitable protocols for preparation of solutions of intact chromosomes delayed the application of flow cytogenetics in plants. This chapter outlines a high-yielding procedure for preparation of solutions of intact mitotic chromosomes from root tips of young seedlings, their analysis using flow cytometry and sorting. Seedlings are easy to handle, root meristems are karyological stable, and high degree of metaphase synchrony can be induced. Chromosomes isolated according to this protocol have well preserved morphology, withstand shearing forces during sorting and their DNA is intact and suitable for a range of applications.
dcterms:title
Chromosome Analysis and Sorting Using Flow Cytometry Chromosome Analysis and Sorting Using Flow Cytometry
skos:prefLabel
Chromosome Analysis and Sorting Using Flow Cytometry Chromosome Analysis and Sorting Using Flow Cytometry
skos:notation
RIV/61389030:_____/11:00375427!RIV12-AV0-61389030
n17:predkladatel
n18:ico%3A61389030
n3:aktivita
n6:P n6:Z
n3:aktivity
P(GA521/07/1573), P(LC06004), Z(AV0Z50380511)
n3:dodaniDat
n10:2012
n3:domaciTvurceVysledku
n4:9544887 n4:5256593 n4:8657572 n4:5207010 n4:1800477
n3:druhVysledku
n14:C
n3:duvernostUdaju
n22:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
190340
n3:idVysledku
RIV/61389030:_____/11:00375427
n3:jazykVysledku
n12:eng
n3:klicovaSlova
Chromosome analysis; flow cytometry; root meristems
n3:klicoveSlovo
n11:root%20meristems n11:flow%20cytometry n11:Chromosome%20analysis
n3:kontrolniKodProRIV
[B32634A98363]
n3:mistoVydani
New Jersey
n3:nazevZdroje
Plant Chromosome Engineering: Methods and Protocols
n3:obor
n9:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetStranKnihy
329
n3:pocetTvurcuVysledku
5
n3:projekt
n7:GA521%2F07%2F1573 n7:LC06004
n3:rokUplatneniVysledku
n10:2011
n3:tvurceVysledku
Kubaláková, Marie Doležel, Jaroslav Šimková, Hana Suchánková, Pavla Číhalíková, Jarmila
n3:zamer
n15:AV0Z50380511
s:numberOfPages
18
n16:doi
10.1007/978-1-61737-957-4_12
n13:hasPublisher
Humana Press
n20:isbn
978-1-61737-956-7