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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F11%3A00368586%21RIV12-AV0-61389030
rdf:type
skos:Concept n15:Vysledek
dcterms:description
This study evaluates the potential of flow cytometry for chromosome sorting in two wild diploid wheats Aegilops umbellulata and Ae. comosa and their natural allotetraploid hybrids Ae. biuncialis and Ae. geniculata. Flow karyotypes obtained after the analysis of DAPI-stained chromosomes were characterized and content of chromosome peaks was determined. Peaks of chromosome 1U could be discriminated in flow karyotypes of Ae. umbellulata and Ae. biuncialis and the chromosome could be sorted with purities exceeding 95%. The remaining chromosomes formed composite peaks and could be sorted in groups of two to four. Twenty four wheat SSR markers were tested for their position on chromosomes of Ae. umbellulata and Ae. comosa using PCR on DNA amplified from flow-sorted chromosomes and genomic DNA of wheat-Ae. geniculata addition lines, respectively. Six SSR markers were located on particular Aegilops chromosomes using sorted chromosomes, thus confirming the usefulness of this approach for physical mapping. This study evaluates the potential of flow cytometry for chromosome sorting in two wild diploid wheats Aegilops umbellulata and Ae. comosa and their natural allotetraploid hybrids Ae. biuncialis and Ae. geniculata. Flow karyotypes obtained after the analysis of DAPI-stained chromosomes were characterized and content of chromosome peaks was determined. Peaks of chromosome 1U could be discriminated in flow karyotypes of Ae. umbellulata and Ae. biuncialis and the chromosome could be sorted with purities exceeding 95%. The remaining chromosomes formed composite peaks and could be sorted in groups of two to four. Twenty four wheat SSR markers were tested for their position on chromosomes of Ae. umbellulata and Ae. comosa using PCR on DNA amplified from flow-sorted chromosomes and genomic DNA of wheat-Ae. geniculata addition lines, respectively. Six SSR markers were located on particular Aegilops chromosomes using sorted chromosomes, thus confirming the usefulness of this approach for physical mapping.
dcterms:title
Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata
skos:prefLabel
Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata
skos:notation
RIV/61389030:_____/11:00368586!RIV12-AV0-61389030
n15:predkladatel
n19:ico%3A61389030
n3:aktivita
n10:P n10:Z
n3:aktivity
P(LC06004), P(OC08025), Z(AV0Z50380511)
n3:cisloPeriodika
11
n3:dodaniDat
n12:2012
n3:domaciTvurceVysledku
n4:5256593 n4:1800477 n4:5207010
n3:druhVysledku
n5:J
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
190341
n3:idVysledku
RIV/61389030:_____/11:00368586
n3:jazykVysledku
n14:eng
n3:klicovaSlova
wheat; chromosome isolation; Aegilops umbellulata
n3:klicoveSlovo
n6:Aegilops%20umbellulata n6:chromosome%20isolation n6:wheat
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[CEF3695CF8FD]
n3:nazevZdroje
PLoS ONE
n3:obor
n8:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n7:OC08025 n7:LC06004
n3:rokUplatneniVysledku
n12:2011
n3:svazekPeriodika
66
n3:tvurceVysledku
Molnár-Láng, M. Kubaláková, Marie Doležel, Jaroslav Molnár, I. Šimková, Hana Cseh, A.
n3:wos
000298162000035
n3:zamer
n13:AV0Z50380511
s:issn
1932-6203
s:numberOfPages
11
n18:doi
10.1371/journal.pone.0027708