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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F11%3A00366403%21RIV12-AV0-61389030
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Mature pollen grain represents a highly desiccated structure with an extremely tough cell wall. Thanks to it, it resists common proteomic protocols. Instead, a robust homogenization has to be performed since proteins are needed to burst out of the cell to be included in the extracted proteome fraction. Here, a novel way of pollen homogenization employing Roche MagNA Lyser Instrument is presented, sparing time and laborious work. However, plant proteomics does not rely solely on perfect homogenization; also the choice of the extraction protocol is of key importance. The composition of the extraction buffer has a decisive influence on which proteome fraction will be extracted. Therefore the second part of our study is dedicated to the comparison of different extraction protocols with respect to subsequent proteomic analyses. Mature pollen grain represents a highly desiccated structure with an extremely tough cell wall. Thanks to it, it resists common proteomic protocols. Instead, a robust homogenization has to be performed since proteins are needed to burst out of the cell to be included in the extracted proteome fraction. Here, a novel way of pollen homogenization employing Roche MagNA Lyser Instrument is presented, sparing time and laborious work. However, plant proteomics does not rely solely on perfect homogenization; also the choice of the extraction protocol is of key importance. The composition of the extraction buffer has a decisive influence on which proteome fraction will be extracted. Therefore the second part of our study is dedicated to the comparison of different extraction protocols with respect to subsequent proteomic analyses.
dcterms:title
Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns
skos:prefLabel
Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns
skos:notation
RIV/61389030:_____/11:00366403!RIV12-AV0-61389030
n12:predkladatel
n13:ico%3A61389030
n3:aktivita
n14:Z n14:P n14:I
n3:aktivity
I, P(GAP501/11/1462), P(OC08011), Z(AV0Z50380511)
n3:cisloPeriodika
3
n3:dodaniDat
n15:2012
n3:domaciTvurceVysledku
n5:8943494 n5:5468582 n5:3853926 n5:5796415
n3:druhVysledku
n17:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
203673
n3:idVysledku
RIV/61389030:_____/11:00366403
n3:jazykVysledku
n18:eng
n3:klicovaSlova
proteomics; Roche MagNA Lyser Instrument; ARABIDOPSIS-THALIANA
n3:klicoveSlovo
n4:ARABIDOPSIS-THALIANA n4:Roche%20MagNA%20Lyser%20Instrument n4:proteomics
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[6249DDD21648]
n3:nazevZdroje
Biologia Plantarum
n3:obor
n20:ED
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n16:OC08011 n16:GAP501%2F11%2F1462
n3:rokUplatneniVysledku
n15:2011
n3:svazekPeriodika
55
n3:tvurceVysledku
Čapková, Věra Honys, David Feciková, Jana Fíla, Jan
n3:wos
000293029800013
n3:zamer
n19:AV0Z50380511
s:issn
0006-3134
s:numberOfPages
8
n6:doi
10.1007/s10535-011-0116-5