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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F10%3A00351018%21RIV11-GA0-61389030
rdf:type
skos:Concept n17:Vysledek
dcterms:description
We describe here a new reversed phase high performance liquid chromatography with mass spectrometry detection method for quantifying intact cytokinin nucleotides in human K-562 leukemia cells. Tandem mass spectrometry (MS/MS) was used to identify the intracellular metabolites (cytokinin mono- di- and tri-phosphorylated nucleotides) in riboside-treated cells. For the protein precipitation and sample preparation, a trichloroacetic acid extraction method is used. Samples are then back-extracted with diethyleter, lyophilized, reconstituted and injected into the LC system. Analytes were quantified in negative selected ion monitoring (SIM) mode using a single quadrupole mass spectrometer. The method was validated in terms of retention time stabilities, limits of detection (LOD), linearity, recovery and analytical accuracy. The developed method was linear in the range of 1-1000 pmol for all studied compounds. The limits of detection for the analytes vary from 0.2 to 0.6 pmol. We describe here a new reversed phase high performance liquid chromatography with mass spectrometry detection method for quantifying intact cytokinin nucleotides in human K-562 leukemia cells. Tandem mass spectrometry (MS/MS) was used to identify the intracellular metabolites (cytokinin mono- di- and tri-phosphorylated nucleotides) in riboside-treated cells. For the protein precipitation and sample preparation, a trichloroacetic acid extraction method is used. Samples are then back-extracted with diethyleter, lyophilized, reconstituted and injected into the LC system. Analytes were quantified in negative selected ion monitoring (SIM) mode using a single quadrupole mass spectrometer. The method was validated in terms of retention time stabilities, limits of detection (LOD), linearity, recovery and analytical accuracy. The developed method was linear in the range of 1-1000 pmol for all studied compounds. The limits of detection for the analytes vary from 0.2 to 0.6 pmol.
dcterms:title
Tandem mass spectrometry identification and LC-MS quantification of intact cytokinin nucleotides in K-562 human leukemia cells Tandem mass spectrometry identification and LC-MS quantification of intact cytokinin nucleotides in K-562 human leukemia cells
skos:prefLabel
Tandem mass spectrometry identification and LC-MS quantification of intact cytokinin nucleotides in K-562 human leukemia cells Tandem mass spectrometry identification and LC-MS quantification of intact cytokinin nucleotides in K-562 human leukemia cells
skos:notation
RIV/61389030:_____/10:00351018!RIV11-GA0-61389030
n3:aktivita
n7:Z n7:P
n3:aktivity
P(1M06030), P(GA301/08/1649), P(GA522/08/0920), P(GD522/08/H003), Z(AV0Z50380511), Z(MSM6198959216)
n3:cisloPeriodika
5
n3:dodaniDat
n12:2011
n3:domaciTvurceVysledku
n4:4712048 n4:2769794 n4:5991803 n4:3686957 n4:4837177 n4:4866029 n4:3063666 n4:8905428
n3:druhVysledku
n14:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
291804
n3:idVysledku
RIV/61389030:_____/10:00351018
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Cytokinins; Nucleotides; HPLC
n3:klicoveSlovo
n10:Cytokinins n10:HPLC n10:Nucleotides
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[6589409D78BC]
n3:nazevZdroje
Analytical and Bioanalytical Chemistry
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
10
n3:projekt
n5:GD522%2F08%2FH003 n5:GA301%2F08%2F1649 n5:1M06030 n5:GA522%2F08%2F0920
n3:rokUplatneniVysledku
n12:2010
n3:svazekPeriodika
398
n3:tvurceVysledku
Novák, Ondřej Hanuš, Jan Tarkowski, Petr Zatloukal, Marek Doležal, Karel Niemann, P. Strnad, Miroslav Voller, Jiří Gahsche, M. C. Béreš, Tibor
n3:wos
000283244500028
n3:zamer
n9:AV0Z50380511 n9:MSM6198959216
s:issn
1618-2642
s:numberOfPages
10