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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F09%3A00337486%21RIV10-AV0-61389030
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Two SOB five-like (SOFL) genes, AtSOFL1 and AtSOFL2, were characterized in Arabidopsis (Arabidopsis thaliana). They act redundantly in regulating specific cytokinin levels. Analysis of the translational fusion AtSOFL1:AtSOFL1-GUS and AtSOFL2:AtSOFL2-GUS indicated that AtSOFL1 and AtSOFL2 are expressed predominantly in vascular tissues of developing leaves, flowers and siliques, but barely detectable in roots and stems. Overexpression of either AtSOFL1 or AtSOFL2 led to increased cytokinin content and corresponding mutant phenotypes for both transgenic seedlings and adult plants. SOFL domain was proved necessary for AtSOFL2´s overexpression phenotypes. Silencing or disrupting either of them caused no obvious developmental defects. The SOFL1-RNAi62/sofl2-1 double mutant accumulated lower levels of trans-zeatin riboside monophosphate (tZRMP) and N6-(2-isopentenyl)adenosine monophosphate (iPRMP), precursors of bioactive cytokinins, and displayed decreased response to exogenous cytokinin. Two SOB five-like (SOFL) genes, AtSOFL1 and AtSOFL2, were characterized in Arabidopsis (Arabidopsis thaliana). They act redundantly in regulating specific cytokinin levels. Analysis of the translational fusion AtSOFL1:AtSOFL1-GUS and AtSOFL2:AtSOFL2-GUS indicated that AtSOFL1 and AtSOFL2 are expressed predominantly in vascular tissues of developing leaves, flowers and siliques, but barely detectable in roots and stems. Overexpression of either AtSOFL1 or AtSOFL2 led to increased cytokinin content and corresponding mutant phenotypes for both transgenic seedlings and adult plants. SOFL domain was proved necessary for AtSOFL2´s overexpression phenotypes. Silencing or disrupting either of them caused no obvious developmental defects. The SOFL1-RNAi62/sofl2-1 double mutant accumulated lower levels of trans-zeatin riboside monophosphate (tZRMP) and N6-(2-isopentenyl)adenosine monophosphate (iPRMP), precursors of bioactive cytokinins, and displayed decreased response to exogenous cytokinin.
dcterms:title
AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis
skos:prefLabel
AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis
skos:notation
RIV/61389030:_____/09:00337486!RIV10-AV0-61389030
n3:aktivita
n11:Z n11:P
n3:aktivity
P(GA206/06/1306), Z(AV0Z50380511)
n3:cisloPeriodika
12
n3:dodaniDat
n10:2010
n3:domaciTvurceVysledku
n8:4739841 n8:4390644 n8:9744886
n3:druhVysledku
n18:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
304362
n3:idVysledku
RIV/61389030:_____/09:00337486
n3:jazykVysledku
n15:eng
n3:klicovaSlova
TRANSGENIC TOBACCO PLANTS; OXIDASE/DEHYDROGENASE GENES; ISOPENTENYL TRANSFERASE
n3:klicoveSlovo
n4:OXIDASE%2FDEHYDROGENASE%20GENES n4:ISOPENTENYL%20TRANSFERASE n4:TRANSGENIC%20TOBACCO%20PLANTS
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[68563725624B]
n3:nazevZdroje
PLoS ONE
n3:obor
n13:ED
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
7
n3:projekt
n17:GA206%2F06%2F1306
n3:rokUplatneniVysledku
n10:2009
n3:svazekPeriodika
4
n3:tvurceVysledku
Vaňková, Radomíra Malbeck, Jiří Dobrev, Petre Neff, M. M. Xu, Y. Chong, K. Zhang, J.
n3:wos
000272829800020
n3:zamer
n14:AV0Z50380511
s:issn
1932-6203
s:numberOfPages
11