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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F09%3A00328620%21RIV10-MZE-61389030
rdf:type
skos:Concept n18:Vysledek
dcterms:description
The complete genomes of three Czech isolates VIRUBRA 1/045, VIRUBRA 1/046, and VIRUBRA 1/047 of Potato leafroll virus (PLRV) were sequenced and compared with 13 complete sequences of PLRV isolates available in GenBank. Among the Czech isolates, VIRUBRA 1/046 and 1/047 showed the highest nucleotide (nt) identity (98.7%). PLRV was the most conserved virus in both open reading frames (ORFs) 3 and 4. The most variable regions were ORFs 0 and Rap1. Interestingly, isolate VIRUBRA 1/045 significantly differed from the other two Czech isolates in ORFs 0 and 1. Moreover, we identified mutations in the amino acid (aa) sequences, which were specific for the Czech isolates. Phylogenetic analysis based on ORF0 showed that the Czech isolates could be classified in two of the three groupings of the phylogenetic tree obtained. This is the first report on sequence analysis of the genome sequences of PLRV isolates from the Czech Republic. The complete genomes of three Czech isolates VIRUBRA 1/045, VIRUBRA 1/046, and VIRUBRA 1/047 of Potato leafroll virus (PLRV) were sequenced and compared with 13 complete sequences of PLRV isolates available in GenBank. Among the Czech isolates, VIRUBRA 1/046 and 1/047 showed the highest nucleotide (nt) identity (98.7%). PLRV was the most conserved virus in both open reading frames (ORFs) 3 and 4. The most variable regions were ORFs 0 and Rap1. Interestingly, isolate VIRUBRA 1/045 significantly differed from the other two Czech isolates in ORFs 0 and 1. Moreover, we identified mutations in the amino acid (aa) sequences, which were specific for the Czech isolates. Phylogenetic analysis based on ORF0 showed that the Czech isolates could be classified in two of the three groupings of the phylogenetic tree obtained. This is the first report on sequence analysis of the genome sequences of PLRV isolates from the Czech Republic.
dcterms:title
Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic
skos:prefLabel
Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic
skos:notation
RIV/61389030:_____/09:00328620!RIV10-MZE-61389030
n4:aktivita
n7:Z n7:P
n4:aktivity
P(1M06030), P(QH71123), Z(AV0Z50380511)
n4:cisloPeriodika
1
n4:dodaniDat
n14:2010
n4:domaciTvurceVysledku
n13:9833242 n13:7888848 n13:3229475
n4:druhVysledku
n15:J
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
341073
n4:idVysledku
RIV/61389030:_____/09:00328620
n4:jazykVysledku
n11:eng
n4:klicovaSlova
Potato leafroll virus; Sequencing; Phylogeny
n4:klicoveSlovo
n12:Sequencing n12:Potato%20leafroll%20virus n12:Phylogeny
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[9DCB69FBE4FC]
n4:nazevZdroje
Virus Genes
n4:obor
n5:EB
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:projekt
n8:QH71123 n8:1M06030
n4:rokUplatneniVysledku
n14:2009
n4:svazekPeriodika
39
n4:tvurceVysledku
Moravec, Tomáš Dědič, P. Plchová, Helena Čeřovská, Noemi
n4:wos
000267299900021
n4:zamer
n17:AV0Z50380511
s:issn
0920-8569
s:numberOfPages
3