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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F08%3A00322259%21RIV09-AV0-61389030
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Byla vyvinuta jednoduchá imunoextrakční mikropurifikační metoda pro extrakci přirozeně se vyskytujících cytokininů kombinovaná s ultra-účinnou kapalinovou chromatografií spojenou s tandemovou hmotnostní spektrometrií. Nové extrakční a purifikační postupy byly optimalizovány na vzorcích topolového listí a analytická přesnost validována na 10-ti denních semenáčcích A. thaliana. Tento přístup může být použit pro rychlou, citlivou kvalitativní a/nebo kvantitativní analýzu víc než 50 přirozených cytokininů v mg množství rostlinných tkání. We have developed a simple, high-throughput batch immunoextraction (IAE) micropurification procedure for extracting a wide range of naturally occurring cytokinins (bases, ribosides, O- and N-glucosides, and nucleotides) from plant tissues in solutions that are compatible with ultra-performance liquid chromatography (UPLC), thereby facilitating sensitive subsequent analysis. The UPLC system was coupled to a tandem quadrupole mass spectrometer (MS/MS) equipped with an electrospray interface (ESI). Small (mg) amounts of tissues were purified by solid-phase extraction (SPE) followed by an immunoaffinity clean-up step and two fast chromatographic separations of most cytokinin metabolites (bases, ribosides, and 9-glucosides in the first, O-glucosides and nucleotides in the second). Using UPLC, the runs were up to 4-fold faster than in standard cytokinin analyses, and both retention times and injection volumes were less variable (RSDs, 0.15–0.3% and 1.0–5.5%, respectively). In multiple reaction monitoring... We have developed a simple, high-throughput batch immunoextraction (IAE) micropurification procedure for extracting a wide range of naturally occurring cytokinins (bases, ribosides, O- and N-glucosides, and nucleotides) from plant tissues in solutions that are compatible with ultra-performance liquid chromatography (UPLC), thereby facilitating sensitive subsequent analysis. The UPLC system was coupled to a tandem quadrupole mass spectrometer (MS/MS) equipped with an electrospray interface (ESI). Small (mg) amounts of tissues were purified by solid-phase extraction (SPE) followed by an immunoaffinity clean-up step and two fast chromatographic separations of most cytokinin metabolites (bases, ribosides, and 9-glucosides in the first, O-glucosides and nucleotides in the second). Using UPLC, the runs were up to 4-fold faster than in standard cytokinin analyses, and both retention times and injection volumes were less variable (RSDs, 0.15–0.3% and 1.0–5.5%, respectively). In multiple reaction monitoring...
dcterms:title
Cytokininová analýza v rostlinných tkáních pomocí ultra-účinné kapalinové chromatografie-elektrosprej tandemové hmotnostní spektrometrie Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry
skos:prefLabel
Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry Cytokininová analýza v rostlinných tkáních pomocí ultra-účinné kapalinové chromatografie-elektrosprej tandemové hmotnostní spektrometrie
skos:notation
RIV/61389030:_____/08:00322259!RIV09-AV0-61389030
n3:aktivita
n15:Z n15:S n15:P
n3:aktivity
P(KAN200380801), S, Z(AV0Z50380511), Z(MSM6198959216)
n3:cisloPeriodika
11
n3:dodaniDat
n16:2009
n3:domaciTvurceVysledku
n4:1263684 n4:2403854 n4:3686957 n4:5991803 n4:4712048
n3:druhVysledku
n18:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
361889
n3:idVysledku
RIV/61389030:_____/08:00322259
n3:jazykVysledku
n7:eng
n3:klicovaSlova
Ultra-performance liquid chromatography (UPLC); Tandem mass spectrometry (MS/MS); Microextraction
n3:klicoveSlovo
n11:Tandem%20mass%20spectrometry%20%28MS%2FMS%29 n11:Ultra-performance%20liquid%20chromatography%20%28UPLC%29 n11:Microextraction
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[C435A0DE3B6A]
n3:nazevZdroje
Phytochemistry
n3:obor
n17:EC
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n6:KAN200380801
n3:rokUplatneniVysledku
n16:2008
n3:svazekPeriodika
69
n3:tvurceVysledku
Hauserová, Eva Doležal, Karel Strnad, Miroslav Novák, Ondřej Amakorová, Petra
n3:wos
000258863100018
n3:zamer
n5:AV0Z50380511 n5:MSM6198959216
s:issn
0031-9422
s:numberOfPages
11