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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F08%3A00307201%21RIV08-AV0-61389030
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Semenáčky tabáku (Nicotiana tabacum var. xanthi) byly ovlivněny roztoky Pb (NO3)2 po dobu 24 h a 7 dní. Průkazné zvýšení poškození DNA, měřeno kometovým testem, bylo prokázáno pouze v kořenech semenáčku. Po kultivaci semenáčků tabáku v pudě kontaminované Pb (NO3)2 po dobu 6 týdnů bylo zvýšení poškození DNA vždy spojeno se silnou toxicitou (inhibice růstu semenáčků, nahnědlé a zdeformované listy). Zvýšení frekvence somatických mutací nebylo prokázáno. Zvýšení poškozené DNA je pravděpodobně podmíněné nekrotickou fragmentací DNA. Tobacco (Nicotiana tabacum L. var. xanthi) seedlings were treated with aqueous solutions of lead nitrate (Pb2+) at concentrations ranging from 0.4 mM to 2.4 mM for 24 h and from 25 μM to 200 μM for 7 days. The DNA damage measured by the comet assay was high in the root nuclei, but in the leaf nuclei a slight but significant increase in DNA damage could be demonstrated only after a 7-day treatment with 200 μM Pb2+. In tobacco plants growing for 6 weeks in soil polluted with Pb2+ severe toxic effects, expressed by the decrease in leaf area, and a slight but significant increase in DNA damage were observed. The tobacco plants with increased levels of DNA damage were severely injured and showed stunted growth, distorted leaves and brown root tips. The frequency of somatic mutations in tobacco plants growing in the Pb2+-polluted soil did not significantly increase. Analytical studies by inductively coupled plasma optical emission spectrometry demonstrate that after a 24-h treatment of tobacco with 2.4 m... Tobacco (Nicotiana tabacum L. var. xanthi) seedlings were treated with aqueous solutions of lead nitrate (Pb2+) at concentrations ranging from 0.4 mM to 2.4 mM for 24 h and from 25 μM to 200 μM for 7 days. The DNA damage measured by the comet assay was high in the root nuclei, but in the leaf nuclei a slight but significant increase in DNA damage could be demonstrated only after a 7-day treatment with 200 μM Pb2+. In tobacco plants growing for 6 weeks in soil polluted with Pb2+ severe toxic effects, expressed by the decrease in leaf area, and a slight but significant increase in DNA damage were observed. The tobacco plants with increased levels of DNA damage were severely injured and showed stunted growth, distorted leaves and brown root tips. The frequency of somatic mutations in tobacco plants growing in the Pb2+-polluted soil did not significantly increase. Analytical studies by inductively coupled plasma optical emission spectrometry demonstrate that after a 24-h treatment of tobacco with 2.4 m...
dcterms:title
Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants Hodnocení poškození DNA a mutagenity po aplikaci olova u rostlin tabaku
skos:prefLabel
Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants Hodnocení poškození DNA a mutagenity po aplikaci olova u rostlin tabaku Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants
skos:notation
RIV/61389030:_____/08:00307201!RIV08-AV0-61389030
n3:strany
186;190
n3:aktivita
n11:V n11:Z n11:P
n3:aktivity
P(GA521/05/0500), V, Z(AV0Z50380511)
n3:cisloPeriodika
2
n3:dodaniDat
n4:2008
n3:domaciTvurceVysledku
n7:9410260
n3:druhVysledku
n5:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
366780
n3:idVysledku
RIV/61389030:_____/08:00307201
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Comet assay; Nicotiana tabacum L. var. xanthi; Single-cell gel electrophoresis; Somatic mutations
n3:klicoveSlovo
n8:Somatic%20mutations n8:Single-cell%20gel%20electrophoresis n8:Comet%20assay n8:Nicotiana%20tabacum%20L.%20var.%20xanthi
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[7EC804BCC3D8]
n3:nazevZdroje
Mutation Research - Genetic Toxicology and Environmental Mutagenesis
n3:obor
n12:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:projekt
n18:GA521%2F05%2F0500
n3:rokUplatneniVysledku
n4:2008
n3:svazekPeriodika
652
n3:tvurceVysledku
Száková, J. Žnidar, I. Gichner, Tomáš
n3:zamer
n10:AV0Z50380511
s:issn
1383-5718
s:numberOfPages
5