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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F07%3A00305980%21RIV08-AV0-61389030
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Cytokinins (CKs) are involved in the regulation of plant development including plastid differentiation and function. Partial location of CK biosynthetic pathways in plastids suggests the importance of CKs for chloroplast development. The impact of genetically modified CK metabolism on endogenous CK, indole-3-acetic acid, and abscisic acid contents in leaves and isolated intact chloroplasts of Nicotiana tabacum was determined by liquid chromatography/mass spectrometry and two-dimensional high-performance liquid chromatography, and alterations in chloroplast ultrastructure by electron microscopy. Ectopic expression of Sho, a gene encoding a Petunia hybrida isopentenyltransferase, was employed to raise CK levels. The increase in CK levels was lower in chloroplasts than in leaves. CK levels were reduced in leaves of tobacco harbouring a CK oxidase/dehydrogenase gene, AtCKX3. The total CK content also decreased in chloroplasts, but CK phosphate levels were higher than in the wild type. In a transformant... Cytokinins (CKs) are involved in the regulation of plant development including plastid differentiation and function. Partial location of CK biosynthetic pathways in plastids suggests the importance of CKs for chloroplast development. The impact of genetically modified CK metabolism on endogenous CK, indole-3-acetic acid, and abscisic acid contents in leaves and isolated intact chloroplasts of Nicotiana tabacum was determined by liquid chromatography/mass spectrometry and two-dimensional high-performance liquid chromatography, and alterations in chloroplast ultrastructure by electron microscopy. Ectopic expression of Sho, a gene encoding a Petunia hybrida isopentenyltransferase, was employed to raise CK levels. The increase in CK levels was lower in chloroplasts than in leaves. CK levels were reduced in leaves of tobacco harbouring a CK oxidase/dehydrogenase gene, AtCKX3. The total CK content also decreased in chloroplasts, but CK phosphate levels were higher than in the wild type. In a transformant... Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco.
dcterms:title
Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco
skos:prefLabel
Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco
skos:notation
RIV/61389030:_____/07:00305980!RIV08-AV0-61389030
n5:strany
637;649
n5:aktivita
n12:V n12:Z n12:P
n5:aktivity
P(GA206/03/0369), P(GA206/06/1306), P(IAA600040612), P(LC06034), V, Z(AV0Z50040702), Z(AV0Z50380511)
n5:cisloPeriodika
3
n5:dodaniDat
n7:2008
n5:domaciTvurceVysledku
n13:4390644 n13:4739841 n13:5683459 n13:9744886 n13:9366849 n13:6071635 n13:2953161
n5:druhVysledku
n14:J
n5:duvernostUdaju
n10:S
n5:entitaPredkladatele
n17:predkladatel
n5:idSjednocenehoVysledku
409126
n5:idVysledku
RIV/61389030:_____/07:00305980
n5:jazykVysledku
n18:eng
n5:klicovaSlova
abscisic acid; auxin; chloroplast ultrastructure
n5:klicoveSlovo
n15:auxin n15:chloroplast%20ultrastructure n15:abscisic%20acid
n5:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n5:kontrolniKodProRIV
[6463010E557C]
n5:nazevZdroje
Journal of Experimental Botany
n5:obor
n16:EF
n5:pocetDomacichTvurcuVysledku
7
n5:pocetTvurcuVysledku
8
n5:projekt
n11:GA206%2F03%2F0369 n11:LC06034 n11:IAA600040612 n11:GA206%2F06%2F1306
n5:rokUplatneniVysledku
n7:2007
n5:svazekPeriodika
58
n5:tvurceVysledku
Vičánková, Anna Malbeck, Jiří Brzobohatý, Břetislav Vaňková, Radomíra Polanská, Lenka Dobrev, Petre Nováková, Marie Macháčková, Ivana
n5:zamer
n6:AV0Z50380511 n6:AV0Z50040702
s:issn
0022-0957
s:numberOfPages
13