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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F07%3A00305830%21RIV08-AV0-61389030
rdf:type
skos:Concept n9:Vysledek
dcterms:description
P5CSF129A cDNA a markerový gen nptII byly použity pro transformaci tabáku (Nicotiana tabacum cv. Bel B and cv. M51) pomocí Agrobacteria tumefaciens klon LBA4404. Dvacet transformovaných rostlin tabáku bylo získáno po transformaci listových disků. Přítomnost transgenu byla potvrzena PCR. Byl studován vliv vodního stresu na růst a fyziologickou odezvu rostlin. Oba kultivary transgenních rostlin, které akumulovaly vysokou hladinu proline, tolerovaly lépe vodní stres, sušina ani obsah chlorofylu nebyly změněny. Za kontrolních i stresových podmínek nebyla pozorována ani předčasná senescence, ani nekrosy listů. Tato studie potvrdila, že P5CSF129A může být potenciálním genem na zlepšení tolerance vůči suchu. P5CSF129A cDNA and the nptII marker gene were used for tobacco (Nicotiana tabacum L. cv. Bel B and cv. M51) transformation via Agrobacterium tumefaciens strain LBA4404. Twenty transformed tobacco plants were obtained after transformation of leaf discs. Presence of the transgene was confirmed by polymerase chain reaction (PCR) analysis. Physiological responses to water stress were compared in transgenic and wild-type tobacco plants. Transgenic plants of both cultivars accumulated high levels of free proline. They did not exhibit dry mass relocation or chlorophyll content reduction. Neither precocious senescence, nor leaf necrosis or morphological changes were observed in control and stress conditions (RWC decrease by 7-8%). Transgenic plants with elevated accumulation of osmoprotectants seem to be better adapted to water stress, providing a perspective for future research of stress effects that have a principle role in the functional activity of plants. This study confirmed P5CSF129A to be a candida... P5CSF129A cDNA and the nptII marker gene were used for tobacco (Nicotiana tabacum L. cv. Bel B and cv. M51) transformation via Agrobacterium tumefaciens strain LBA4404. Twenty transformed tobacco plants were obtained after transformation of leaf discs. Presence of the transgene was confirmed by polymerase chain reaction (PCR) analysis. Physiological responses to water stress were compared in transgenic and wild-type tobacco plants. Transgenic plants of both cultivars accumulated high levels of free proline. They did not exhibit dry mass relocation or chlorophyll content reduction. Neither precocious senescence, nor leaf necrosis or morphological changes were observed in control and stress conditions (RWC decrease by 7-8%). Transgenic plants with elevated accumulation of osmoprotectants seem to be better adapted to water stress, providing a perspective for future research of stress effects that have a principle role in the functional activity of plants. This study confirmed P5CSF129A to be a candida...
dcterms:title
Transformované rostliny tabáku se zvýšenou tolerancí vůči suchu Transformed tobacco plants with increased tolerance to drought Transformed tobacco plants with increased tolerance to drought
skos:prefLabel
Transformed tobacco plants with increased tolerance to drought Transformed tobacco plants with increased tolerance to drought Transformované rostliny tabáku se zvýšenou tolerancí vůči suchu
skos:notation
RIV/61389030:_____/07:00305830!RIV08-AV0-61389030
n4:strany
505;511
n4:aktivita
n11:V n11:Z
n4:aktivity
V, Z(AV0Z50380511)
n4:cisloPeriodika
4
n4:dodaniDat
n7:2008
n4:domaciTvurceVysledku
n15:9744886
n4:druhVysledku
n14:J
n4:duvernostUdaju
n10:S
n4:entitaPredkladatele
n13:predkladatel
n4:idSjednocenehoVysledku
455551
n4:idVysledku
RIV/61389030:_____/07:00305830
n4:jazykVysledku
n17:eng
n4:klicovaSlova
Nicotiana tabacum L; pigment; proline
n4:klicoveSlovo
n6:pigment n6:Nicotiana%20tabacum%20L n6:proline
n4:kodStatuVydavatele
ZA - Jihoafrická republika
n4:kontrolniKodProRIV
[98A893ED24C1]
n4:nazevZdroje
South African Journal of Botany
n4:obor
n12:EB
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
8
n4:rokUplatneniVysledku
n7:2007
n4:svazekPeriodika
73
n4:tvurceVysledku
Dragúňová, M. Lichtnerová, H. Červená, V. Jureková, Z. Gubiš, J. Dokupil, T. Vaňková, Radomíra Hudcovicová, M.
n4:zamer
n16:AV0Z50380511
s:issn
0254-6299
s:numberOfPages
7