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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F07%3A00099494%21RIV08-AV0-61389030
rdf:type
skos:Concept n11:Vysledek
dcterms:description
Intergenová oblast psbA-trnH v chloroplastovém genomu krytosemenných rostlin je velmi variabilní a hojně využívaná při studiu populací. Byla navržena k použití pro „barcoding“, tedy k určování druhů podle DNA sekvence. Tato oblast obsahuje psbA 3’UTR přepisovaný úsek. Srovnali jsme sekvenci a sekundární strukturu RNA v této oblasti u krytosemenných rostlin a nalezli konzervované motivy odpovídající stonkům vlásenek dvouřetězcové RNA a konsensus sekvenci TTAGTGTATA, která nevykazovala výraznou sekundární strukturu. Srovnání chloroplastových UTR pomůže pomoci najít evolučně konzervované elementy a pochopit evoluci nekódujících oblastí chloroplastov0ho genomu. The psbA-trnH intergenic region is among the most variable regions in the angiosperm chloroplast genome. It is a popular tool for plant population genetics and species level phylogenetics and has been proposed as suitable for DNA barcoding studies. This region contains two parts differing in their evolutionary conservation: 1) the psbA 3’UTR (untranslated region) and 2) the psbA-trnH intergenic non-transcribed spacer. We compared the sequence and RNA secondary structure of the psbA 3' UTR across angiosperms and found consensus motifs corresponding to the stem portions of the RNA stem-loop structures and a consensus TTAGTGTATA box. The psbA-trnH spacer exhibited patterns explicable by the independent evolution of large inversions in the psbA 3’UTR and mutational hot spots in the remaining portion of the psbA-trnH spacer. We conclude that a comparison of chloroplast UTRs across a angiosperms offer clues to the identity of putative regulatory elements and information about selective constraints impos... The psbA-trnH intergenic region is among the most variable regions in the angiosperm chloroplast genome. It is a popular tool for plant population genetics and species level phylogenetics and has been proposed as suitable for DNA barcoding studies. This region contains two parts differing in their evolutionary conservation: 1) the psbA 3’UTR (untranslated region) and 2) the psbA-trnH intergenic non-transcribed spacer. We compared the sequence and RNA secondary structure of the psbA 3' UTR across angiosperms and found consensus motifs corresponding to the stem portions of the RNA stem-loop structures and a consensus TTAGTGTATA box. The psbA-trnH spacer exhibited patterns explicable by the independent evolution of large inversions in the psbA 3’UTR and mutational hot spots in the remaining portion of the psbA-trnH spacer. We conclude that a comparison of chloroplast UTRs across a angiosperms offer clues to the identity of putative regulatory elements and information about selective constraints impos...
dcterms:title
The architecture of the chloroplast trnH-psbA non-coding region in angiosperms Architektura nekódující chloroplastové oblasti psbA-trnH u krytosemenných rostlin The architecture of the chloroplast trnH-psbA non-coding region in angiosperms
skos:prefLabel
The architecture of the chloroplast trnH-psbA non-coding region in angiosperms Architektura nekódující chloroplastové oblasti psbA-trnH u krytosemenných rostlin The architecture of the chloroplast trnH-psbA non-coding region in angiosperms
skos:notation
RIV/61389030:_____/07:00099494!RIV08-AV0-61389030
n3:strany
235;256
n3:aktivita
n5:P n5:V n5:Z
n3:aktivity
P(LC06004), V, Z(AV0Z50380511)
n3:cisloPeriodika
1-4
n3:dodaniDat
n12:2008
n3:domaciTvurceVysledku
n4:5953685
n3:druhVysledku
n18:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
410675
n3:idVysledku
RIV/61389030:_____/07:00099494
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Chloroplast DNA; psbA-trnH intergenic region; Silene; deletions; insertions and inversions in stem-loop region; psbA 3´untranslated region; RNA secondary structure
n3:klicoveSlovo
n8:insertions%20and%20inversions%20in%20stem-loop%20region n8:psbA%203%C2%B4untranslated%20region n8:psbA-trnH%20intergenic%20region n8:RNA%20secondary%20structure n8:deletions n8:Chloroplast%20DNA n8:Silene
n3:kodStatuVydavatele
AT - Rakouská republika
n3:kontrolniKodProRIV
[00306D191104]
n3:nazevZdroje
Plant Systematics and Evolution
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
2
n3:projekt
n10:LC06004
n3:rokUplatneniVysledku
n12:2007
n3:svazekPeriodika
268
n3:tvurceVysledku
Olson, M. S. Štorchová, Helena
n3:zamer
n9:AV0Z50380511
s:issn
0378-2697
s:numberOfPages
22