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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F06%3A00041855%21RIV07-AV0-61389030
rdf:type
n7:Vysledek skos:Concept
dcterms:description
The gene encoding the triple gene protein 1 (TGBp1) of Potato mop-top protein (PMTV) was cloned into expression vector pQE32 tagging the protein with 6xHis on the N-terminus. When the gene was enshortened on its 3´-end by two different restriction digestions, efficient and high yield bacterial expression was achieved in both cases, as shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PHAGE) and Western blot. One of these two constructs was used for raising rabbit polyclonal antibodies. The obtained sera and antibodies were tested for the detection of PMTV in laboratory host Nicotiana bethamiana and natural host Solanum tuberosum by enzyme-linked immunosorbent assay (ELISA) as well as by Western blota. The obtainad antisera are more suitable for Western blot analysis of infected plants than for ELSA. The gene encoding the triple gene protein 1 (TGBp1) of Potato mop-top protein (PMTV) was cloned into expression vector pQE32 tagging the protein with 6xHis on the N-terminus. When the gene was enshortened on its 3´-end by two different restriction digestions, efficient and high yield bacterial expression was achieved in both cases, as shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PHAGE) and Western blot. One of these two constructs was used for raising rabbit polyclonal antibodies. The obtained sera and antibodies were tested for the detection of PMTV in laboratory host Nicotiana bethamiana and natural host Solanum tuberosum by enzyme-linked immunosorbent assay (ELISA) as well as by Western blota. The obtainad antisera are more suitable for Western blot analysis of infected plants than for ELSA. Produkce polyklonálních protilátek proti rekombinantnímu nestrukturálnímu proteinu 1 z „triple gene blocku“ mop-top viru bramboru. Gen kodující protein 1 z „triple gene block“ (TGBp1) mop-top viru bramboru byl klonován v expresivním vektoru pQE32. Pomocí postupného zkracování genu na jeho 3 konci dvěma různými restrikčními digescemi bylo u obou konstruktů dosaženo vysoké bakteriální exprese. Jeden z těchto konstruktů byl použit pro produkci králičích polyklonálních protilátek. Získaná antiséra a protilátky byly testovány na přítomnost PMTV v laboratorním hostiteli Nicotina bentamiana a na přirozeném hostiteli Solanum tuberosum . Získaná antiséra jsou vhodnější pro identifikaci infekčních rostlin metodou Western blotu než pro metodu ELISA.
dcterms:title
Produkce polyklonálních protilátek proti rekombinantnímu nestrukturálnímu proteinu 1 z „triple gene blocku“ mop-top viru bramboru Production of Polyclonal Antiobies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein l Production of Polyclonal Antiobies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein l
skos:prefLabel
Production of Polyclonal Antiobies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein l Production of Polyclonal Antiobies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein l Produkce polyklonálních protilátek proti rekombinantnímu nestrukturálnímu proteinu 1 z „triple gene blocku“ mop-top viru bramboru
skos:notation
RIV/61389030:_____/06:00041855!RIV07-AV0-61389030
n4:strany
422;427
n4:aktivita
n9:Z n9:P
n4:aktivity
P(GA522/04/1329), Z(AV0Z50380511)
n4:cisloPeriodika
-
n4:dodaniDat
n16:2007
n4:domaciTvurceVysledku
n6:3229475 n6:6479383 n6:5470056
n4:druhVysledku
n15:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n8:predkladatel
n4:idSjednocenehoVysledku
495189
n4:idVysledku
RIV/61389030:_____/06:00041855
n4:jazykVysledku
n12:eng
n4:klicovaSlova
Potato mop-top virus; recombinant protein; triple gene block; polyclonal antibodies
n4:klicoveSlovo
n5:polyclonal%20antibodies n5:Potato%20mop-top%20virus n5:triple%20gene%20block n5:recombinant%20protein
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[428C2F6924C0]
n4:nazevZdroje
Journal of Phytopathology - Phytopathologische Zeitschrift
n4:obor
n11:EB
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
3
n4:projekt
n14:GA522%2F04%2F1329
n4:rokUplatneniVysledku
n16:2006
n4:svazekPeriodika
154
n4:tvurceVysledku
Filigarová, Marie Pečenková, Tamara Čeřovská, Noemi
n4:zamer
n18:AV0Z50380511
s:issn
0931-1785
s:numberOfPages
6