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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F05%3A00081533%21RIV07-AV0-61389030
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Průtoková cytometrie a třídění byly aplikovány při řešení celého spektra problémů rostlinných biotechnologií a genomiky. Možnosti aplikace průtokové cytometrie byly značně rozšířeny vývojem metod pro expresi spontánně fluorescenčních markerů v transgenních organismech. Z nich zelený fluorescenční protein (GFP) a jeho deriváty jsou nejznámější a nejdůležitější. Metody analýzy a třídění průtokovou cytometrií byly rovněž vyvinuty pro buněčné organely, včetně jader, mitochondrií a chloroplastů a využívají vlastní fluorescence (chloroplasty), in vivo expresi GFP, nebo barvení fluorescenčními barvivy. Flow cytometry and cell sorting has been applied to a wide variety of different problems in plant biotechnology and genomics. Applications of flow cytometry and sorting have been particularly boosted by the development of methods for expression of intrinsically fluorescent markers within transgenic organisms, of which the Green Fluorescent Protein and its derivatives, are the most popular and important. Flow cytometric analysis and sorting applications have also been developed for use with subcellular organelles, including nuclei, mitochondria, and chloroplasts, based either on intrinsic fluorescence (chloroplasts), in vivo GFP expression and targeting, or staining with particular fluorochromes. Flow cytometry and cell sorting has been applied to a wide variety of different problems in plant biotechnology and genomics. Applications of flow cytometry and sorting have been particularly boosted by the development of methods for expression of intrinsically fluorescent markers within transgenic organisms, of which the Green Fluorescent Protein and its derivatives, are the most popular and important. Flow cytometric analysis and sorting applications have also been developed for use with subcellular organelles, including nuclei, mitochondria, and chloroplasts, based either on intrinsic fluorescence (chloroplasts), in vivo GFP expression and targeting, or staining with particular fluorochromes.
dcterms:title
Flow cytometry and cell sorting in plant biotechology Průtoková cytometrie a třídění buněk v rostlinné biotechnologii Flow cytometry and cell sorting in plant biotechology
skos:prefLabel
Flow cytometry and cell sorting in plant biotechology Flow cytometry and cell sorting in plant biotechology Průtoková cytometrie a třídění buněk v rostlinné biotechnologii
skos:notation
RIV/61389030:_____/05:00081533!RIV07-AV0-61389030
n3:strany
291;322
n3:aktivita
n4:Z n4:P
n3:aktivity
P(GA204/04/1207), P(GA521/03/0595), P(GA521/04/0607), Z(AV0Z50380511)
n3:dodaniDat
n8:2007
n3:domaciTvurceVysledku
n13:1800477 n13:6390471
n3:druhVysledku
n12:C
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
521816
n3:idVysledku
RIV/61389030:_____/05:00081533
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Flow cytometry; cell sorting; plant biotechnology
n3:klicoveSlovo
n5:Flow%20cytometry n5:cell%20sorting n5:plant%20biotechnology
n3:kontrolniKodProRIV
[BEF039248E9E]
n3:mistoVydani
Oxford
n3:nazevZdroje
Flow Cytometry for Biotechnology
n3:obor
n10:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n9:GA521%2F03%2F0595 n9:GA204%2F04%2F1207 n9:GA521%2F04%2F0607
n3:rokUplatneniVysledku
n8:2005
n3:tvurceVysledku
Bartoš, Jan Doležel, Jaroslav Galbraith, D. W.
n3:zamer
n11:AV0Z50380511
s:numberOfPages
32
n14:hasPublisher
Oxford University Press
n20:isbn
0-19-515234-4