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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F03%3A56033097%21RIV%2F2004%2FGA0%2FA56004%2FN
rdf:type
n7:Vysledek skos:Concept
dcterms:description
Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be disrupted by several other adenine- but not guanine-based purine derivatives. The observed interaction with cytokinins is discussed in view of a putative role for adenosine kinase in TBY-2 cytokinin metabolism. The presented results show for the first time a plant adenosine kinase affinity-purified to homogeneity that was identified by primary structure analysis. Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be disrupted by several other adenine- but not guanine-based purine derivatives. The observed interaction with cytokinins is discussed in view of a putative role for adenosine kinase in TBY-2 cytokinin metabolism. The presented results show for the first time a plant adenosine kinase affinity-purified to homogeneity that was identified by primary structure analysis.
dcterms:title
Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase. Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.
skos:prefLabel
Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase. Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.
skos:notation
RIV/61389030:_____/03:56033097!RIV/2004/GA0/A56004/N
n3:strany
63;66
n3:aktivita
n15:P n15:Z
n3:aktivity
P(GA301/02/0475), Z(AV0Z5038910), Z(MSM 153100008)
n3:cisloPeriodika
1/3
n3:dodaniDat
n16:2004
n3:domaciTvurceVysledku
n8:9896821 n8:4712048
n3:druhVysledku
n13:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
602573
n3:idVysledku
RIV/61389030:_____/03:56033097
n3:jazykVysledku
n6:eng
n3:klicovaSlova
Adenosine kinase; Tobacco Bright Yellow 2; Cytokinin
n3:klicoveSlovo
n5:Tobacco%20Bright%20Yellow%202 n5:Adenosine%20kinase n5:Cytokinin
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[20CB01950B2F]
n3:nazevZdroje
FEBS Letters
n3:obor
n18:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
5
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n10:GA301%2F02%2F0475
n3:rokUplatneniVysledku
n16:2003
n3:svazekPeriodika
533
n3:tvurceVysledku
Witters, E. Laukens, K. Lenobel, René Onckelen, H. Strnad, Miroslav
n3:zamer
n4:AV0Z5038910 n4:MSM%20153100008
s:issn
0014-5793
s:numberOfPages
4