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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F03%3A56033053%21RIV%2F2004%2FGA0%2FA56004%2FN
rdf:type
skos:Concept n11:Vysledek
dcterms:description
A mouse hybridoma was cultured either in standard serum-free medium, or in medium diluted to 30%, in which the cells underwent nutritional stress. Steady-state parameters of semicontinuous cultures conducted at 24-epibrassinolide concentrations from 10-16 to 10-9 mol l-1 were evaluated. Typical effects of the agent found both in standard and in diluted media were (i) increase in the value of mitochondrial membrane potential, (ii), drop of intracellular antibody level, (iii) increase in the fraction of the cells in the G0/G1 phase, and (iv) decrease in the fraction of the cells in the S phase. Alleviation of nutritional stress manifested itself in cultures conducted in diluted media. At the lowest brassinosteroid concentrations, the number of steroid molecules in the culture was of the same order of magnitude as the number of viable cells in the culture. This implies involvement of a complex cascade mechanism, through which the steroid molecule induces alterations in gene expression leading finally to A mouse hybridoma was cultured either in standard serum-free medium, or in medium diluted to 30%, in which the cells underwent nutritional stress. Steady-state parameters of semicontinuous cultures conducted at 24-epibrassinolide concentrations from 10-16 to 10-9 mol l-1 were evaluated. Typical effects of the agent found both in standard and in diluted media were (i) increase in the value of mitochondrial membrane potential, (ii), drop of intracellular antibody level, (iii) increase in the fraction of the cells in the G0/G1 phase, and (iv) decrease in the fraction of the cells in the S phase. Alleviation of nutritional stress manifested itself in cultures conducted in diluted media. At the lowest brassinosteroid concentrations, the number of steroid molecules in the culture was of the same order of magnitude as the number of viable cells in the culture. This implies involvement of a complex cascade mechanism, through which the steroid molecule induces alterations in gene expression leading finally to
dcterms:title
24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA. 24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA.
skos:prefLabel
24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA. 24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA.
skos:notation
RIV/61389030:_____/03:56033053!RIV/2004/GA0/A56004/N
n3:strany
2190;2200
n3:aktivita
n7:P n7:Z
n3:aktivity
P(GA203/01/0083), P(OC 844.10), Z(AV0Z4055905), Z(AV0Z5038910)
n3:cisloPeriodika
11
n3:dodaniDat
n5:2004
n3:domaciTvurceVysledku
n16:5671140
n3:druhVysledku
n8:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
636792
n3:idVysledku
RIV/61389030:_____/03:56033053
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Steroids; Brassinosteroids; 24-Epibrassinolides
n3:klicoveSlovo
n13:Steroids n13:Brassinosteroids n13:24-Epibrassinolides
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[A458ECC81325]
n3:nazevZdroje
Collection of Czechoslovak Chemical Communications
n3:obor
n17:CE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n14:GA203%2F01%2F0083 n14:OC%20844.10
n3:rokUplatneniVysledku
n5:2003
n3:svazekPeriodika
68
n3:tvurceVysledku
Franěk, František Eckschlager, T. Kohout, Ladislav
n3:zamer
n9:AV0Z4055905 n9:AV0Z5038910
s:issn
0010-0765
s:numberOfPages
11