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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F02%3A56023055%21RIV%2F2003%2FGA0%2FA56003%2FN
rdf:type
skos:Concept n16:Vysledek
dcterms:description
A single-step, highly specific and easy-to-use method was developed for isolation and purification of melatonin from complex biological matrices. Polyclonal antibodies highly specific against melatonin (with cross-reactivities with related compounds below 0.02%, except for 6-hydroxymelatonin) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of immunoaffinity gel. Melatonin recovery by the immunoaffinity method was ~95%, allowing single-step processing of samples prior to electrospray HPLCůMS analysis (with detection limit 10 fmol). The method was successfully used for determining melatonin in human serum and turned out to be better than the non-specific solid-phase extraction published earlier. A single-step, highly specific and easy-to-use method was developed for isolation and purification of melatonin from complex biological matrices. Polyclonal antibodies highly specific against melatonin (with cross-reactivities with related compounds below 0.02%, except for 6-hydroxymelatonin) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of immunoaffinity gel. Melatonin recovery by the immunoaffinity method was ~95%, allowing single-step processing of samples prior to electrospray HPLCůMS analysis (with detection limit 10 fmol). The method was successfully used for determining melatonin in human serum and turned out to be better than the non-specific solid-phase extraction published earlier.
dcterms:title
Isolation of melatonin by immunoaffinity chromatography. Isolation of melatonin by immunoaffinity chromatography.
skos:prefLabel
Isolation of melatonin by immunoaffinity chromatography. Isolation of melatonin by immunoaffinity chromatography.
skos:notation
RIV/61389030:_____/02:56023055!RIV/2003/GA0/A56003/N
n3:strany
9;15
n3:aktivita
n8:P n8:Z
n3:aktivity
P(GA301/02/0475), P(OC 844.10), Z(AV0Z5038910), Z(MSM 153100008)
n3:cisloPeriodika
1
n3:dodaniDat
n11:2003
n3:domaciTvurceVysledku
n6:3491498 n6:9896821 n6:7416113 n6:4712048
n3:druhVysledku
n10:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
649797
n3:idVysledku
RIV/61389030:_____/02:56023055
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Melatonin
n3:klicoveSlovo
n12:Melatonin
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[84B26B93C32A]
n3:nazevZdroje
Journal of Chromatography. B - Biomedical Sciences and Applications
n3:obor
n13:CE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n4:GA301%2F02%2F0475 n4:OC%20844.10
n3:rokUplatneniVysledku
n11:2002
n3:svazekPeriodika
25
n3:tvurceVysledku
Strnad, Miroslav Rolčík, Jakub Siglerová, Věra Lenobel, René
n3:zamer
n5:AV0Z5038910 n5:MSM%20153100008
s:issn
0378-4347
s:numberOfPages
7