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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F02%3A56023045%21RIV%2F2003%2FGA0%2FA56003%2FN
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Two 2,6,9-trisubstituted purine derivatives, 9-isopropyl-2,6-bis[(4-methoxybenzyl)amino]-9H-purine (myoseverin, PMYO, 1) and 9-isopropyl-2,6-bis[(2-methoxybenzyl)amino]-9H-purine (OMYO, 2), and two 6,9-disubstituted derivatives, 9-isopropyl-6-[(4-methoxybenzyl)amino]-9H-purine (3) and 9-isopropyl-6-[(2-methoxybenzyl)amino]-9H-purine (4), were synthesized with the aim to examine their cell proliferation inhibiting activity, and possible additional effects in cultures of hybridoma cells producing monoclonal antibody. The substances were tested over a concentration range from 0.003 to 30 mmol l-1. The most active compound 1 caused a total loss of cell viability at 1 mmol l-1, while its isomer 2 showed the same effect at 10 mmol l-1 concentration. In the presence of compound 1, but not of compound 2, the character of the cell cycle phases profile changed dramatically, most cells being arrested in the G2/M phase. At intermediate concentrations of compound 2 a substantially higher viable cell concentration Two 2,6,9-trisubstituted purine derivatives, 9-isopropyl-2,6-bis[(4-methoxybenzyl)amino]-9H-purine (myoseverin, PMYO, 1) and 9-isopropyl-2,6-bis[(2-methoxybenzyl)amino]-9H-purine (OMYO, 2), and two 6,9-disubstituted derivatives, 9-isopropyl-6-[(4-methoxybenzyl)amino]-9H-purine (3) and 9-isopropyl-6-[(2-methoxybenzyl)amino]-9H-purine (4), were synthesized with the aim to examine their cell proliferation inhibiting activity, and possible additional effects in cultures of hybridoma cells producing monoclonal antibody. The substances were tested over a concentration range from 0.003 to 30 mmol l-1. The most active compound 1 caused a total loss of cell viability at 1 mmol l-1, while its isomer 2 showed the same effect at 10 mmol l-1 concentration. In the presence of compound 1, but not of compound 2, the character of the cell cycle phases profile changed dramatically, most cells being arrested in the G2/M phase. At intermediate concentrations of compound 2 a substantially higher viable cell concentration
dcterms:title
Effect of the purine derivative myoseverin and of its analogues on cultured hybridoma cells. Effect of the purine derivative myoseverin and of its analogues on cultured hybridoma cells.
skos:prefLabel
Effect of the purine derivative myoseverin and of its analogues on cultured hybridoma cells. Effect of the purine derivative myoseverin and of its analogues on cultured hybridoma cells.
skos:notation
RIV/61389030:_____/02:56023045!RIV/2003/GA0/A56003/N
n3:strany
257;266
n3:aktivita
n15:Z n15:P
n3:aktivity
P(GA301/02/0475), P(GA303/02/0875), P(OC 844.10), Z(AV0Z5038910)
n3:cisloPeriodika
2
n3:dodaniDat
n7:2003
n3:domaciTvurceVysledku
n11:7416113 n11:5671140 n11:9130241 n11:4712048
n3:druhVysledku
n16:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
644354
n3:idVysledku
RIV/61389030:_____/02:56023045
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Purines; CDK Inhibitors; Cytokinins
n3:klicoveSlovo
n13:CDK%20Inhibitors n13:Purines n13:Cytokinins
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[BDCF9DC16C47]
n3:nazevZdroje
Collection of Czechoslovak Chemical Communications
n3:obor
n12:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n4:OC%20844.10 n4:GA301%2F02%2F0475 n4:GA303%2F02%2F0875
n3:rokUplatneniVysledku
n7:2002
n3:svazekPeriodika
67
n3:tvurceVysledku
Havlíček, Libor Eckschlager, T. Siglerová, Věra Franěk, František Weigl, E. Strnad, Miroslav
n3:zamer
n17:AV0Z5038910
s:issn
0010-0765
s:numberOfPages
10