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Statements

Subject Item
n2:RIV%2F61389030%3A_____%2F02%3A56023014%21RIV%2F2003%2FAV0%2FA56003%2FN
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Previously, we reported on the development of procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) in bread wheat. That study indicated the possibility of sorting large quantities of intact chromosomes, and their suitability for analysis at the molecular level. However, due to the lack of sufficient differences in size between individual chromosomes, only chromosome 3B could be sorted into a high-purity fraction. The present study aimed to identify wheat stocks that could be used to sort other chromosomes. An analysis of 58 varieties and landraces demonstrated a remarkable reproducibility and sensitivity of flow cytometry for the detection of numerical and structural chromosome changes. Changes in flow karyotype, diagnostic for the presence of the 1BL-1RS transloca-tion, have been found and lines from which translocation chromosomes 5BL-7BL and 4AL-4AS-5BL could be sorted have been identified. Furthermore, wheat lines have been identified which can be used for sortin Previously, we reported on the development of procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) in bread wheat. That study indicated the possibility of sorting large quantities of intact chromosomes, and their suitability for analysis at the molecular level. However, due to the lack of sufficient differences in size between individual chromosomes, only chromosome 3B could be sorted into a high-purity fraction. The present study aimed to identify wheat stocks that could be used to sort other chromosomes. An analysis of 58 varieties and landraces demonstrated a remarkable reproducibility and sensitivity of flow cytometry for the detection of numerical and structural chromosome changes. Changes in flow karyotype, diagnostic for the presence of the 1BL-1RS transloca-tion, have been found and lines from which translocation chromosomes 5BL-7BL and 4AL-4AS-5BL could be sorted have been identified. Furthermore, wheat lines have been identified which can be used for sortin
dcterms:title
Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L). Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L).
skos:prefLabel
Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L). Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L).
skos:notation
RIV/61389030:_____/02:56023014!RIV/2003/AV0/A56003/N
n3:strany
1362;1372
n3:aktivita
n14:Z n14:P
n3:aktivity
P(IAA6038204), P(IBS5038104), Z(AV0Z5038910)
n3:cisloPeriodika
N/A
n3:dodaniDat
n6:2003
n3:domaciTvurceVysledku
n5:9606599 n5:5256593 n5:5207010 n5:9544887 n5:1800477
n3:druhVysledku
n18:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
646340
n3:idVysledku
RIV/61389030:_____/02:56023014
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Chromosome isolation; Chromosome sorting; Flow cytometry
n3:klicoveSlovo
n9:Chromosome%20isolation n9:Chromosome%20sorting n9:Flow%20cytometry
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[E2A4CE7F59F9]
n3:nazevZdroje
Theoretical and Applied Genetics
n3:obor
n15:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n4:IBS5038104 n4:IAA6038204
n3:rokUplatneniVysledku
n6:2002
n3:svazekPeriodika
104
n3:tvurceVysledku
Vrána, Jan Doleželová, Marie Čihalíková, Jarmila Šimková, Hana Doležel, Jaroslav
n3:zamer
n7:AV0Z5038910
s:issn
0040-5752
s:numberOfPages
11