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Statements

Subject Item
n2:RIV%2F61389013%3A_____%2F14%3A00427702%21RIV15-GA0-61389013
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Label-free affinity biosensors offer a promising platform for the development of a new generation of medical diagnostic technologies. Nevertheless, when such sensors are used in complex biological media, adsorption of non-targeted medium components prevents the specific detection of the analyte. In this work, we introduce for the first time a biosensor assay based on surface plasmon resonance (SPR) capable of diagnosing different stages of Epstein–Barr virus (EBV) infections in clinical serum samples. This was achieved by simultaneous detection of the antibodies against three different antigens present in the virus. To prevent the interference of the fouling from serum during the measurement, the SPR chips were coated by an antifouling layer of a polymer brush of poly[oligo(ethylene glycol) methacrylate] grown by surface-initiated atom transfer radical polymerization. The bioreceptors were then attached via hybridization of complementary oligonucleotides. This allowed the sensor surface to be regenerated after measurement by disrupting the complementary pairs above the oligonucleotides' melting temperature and attaching new bioreceptors. In this way, the same sensing surface could be used repeatedly. The procedure used in this work will serve as a prototype strategy for the development of label-free affinity biosensors for diagnostics in blood serum or plasma samples. This is the first example of detection of marker of a disease in clinical serum samples by an optical affinity biosensor. Label-free affinity biosensors offer a promising platform for the development of a new generation of medical diagnostic technologies. Nevertheless, when such sensors are used in complex biological media, adsorption of non-targeted medium components prevents the specific detection of the analyte. In this work, we introduce for the first time a biosensor assay based on surface plasmon resonance (SPR) capable of diagnosing different stages of Epstein–Barr virus (EBV) infections in clinical serum samples. This was achieved by simultaneous detection of the antibodies against three different antigens present in the virus. To prevent the interference of the fouling from serum during the measurement, the SPR chips were coated by an antifouling layer of a polymer brush of poly[oligo(ethylene glycol) methacrylate] grown by surface-initiated atom transfer radical polymerization. The bioreceptors were then attached via hybridization of complementary oligonucleotides. This allowed the sensor surface to be regenerated after measurement by disrupting the complementary pairs above the oligonucleotides' melting temperature and attaching new bioreceptors. In this way, the same sensing surface could be used repeatedly. The procedure used in this work will serve as a prototype strategy for the development of label-free affinity biosensors for diagnostics in blood serum or plasma samples. This is the first example of detection of marker of a disease in clinical serum samples by an optical affinity biosensor.
dcterms:title
Diagnosis of Epstein-Barr virus infection in clinical serum samples by an SPR biosensor assay Diagnosis of Epstein-Barr virus infection in clinical serum samples by an SPR biosensor assay
skos:prefLabel
Diagnosis of Epstein-Barr virus infection in clinical serum samples by an SPR biosensor assay Diagnosis of Epstein-Barr virus infection in clinical serum samples by an SPR biosensor assay
skos:notation
RIV/61389013:_____/14:00427702!RIV15-GA0-61389013
n3:aktivita
n16:P n16:I
n3:aktivity
I, P(ED1.1.00/02.0109), P(EE2.3.30.0029), P(GBP205/12/G118)
n3:cisloPeriodika
15 May
n3:dodaniDat
n4:2015
n3:domaciTvurceVysledku
n5:1778013 Rodriguez-Emmenegger, Cesar n5:2239272 de los Santos Pereira, Andres
n3:druhVysledku
n18:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
11137
n3:idVysledku
RIV/61389013:_____/14:00427702
n3:jazykVysledku
n13:eng
n3:klicovaSlova
surface plasmon resonance biosensor; real time diagnostics; Epstein–Barr virus infection
n3:klicoveSlovo
n11:Epstein%E2%80%93Barr%20virus%20infection n11:real%20time%20diagnostics n11:surface%20plasmon%20resonance%20biosensor
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[7F4DFE2366A9]
n3:nazevZdroje
Biosensors and Bioelectronics
n3:obor
n9:CE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
7
n3:projekt
n7:ED1.1.00%2F02.0109 n7:GBP205%2F12%2FG118 n7:EE2.3.30.0029
n3:rokUplatneniVysledku
n4:2014
n3:svazekPeriodika
55
n3:tvurceVysledku
Boltovets, P. M. Bědajánková, A. Rodriguez-Emmenegger, Cesar Jinoch, P. Riedel, Tomáš de los Santos Pereira, Andres Brynda, Eduard
n3:wos
000332426100044
s:issn
0956-5663
s:numberOfPages
7
n15:doi
10.1016/j.bios.2013.12.011