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Statements

Subject Item
n2:RIV%2F61389013%3A_____%2F14%3A00427443%21RIV15-AV0-61389013
rdf:type
skos:Concept n12:Vysledek
dcterms:description
In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells—specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide-PEG-hydrazide, and subsequently anti-epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti-EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied in an Ephesia chip and an evaluation was performed by the MCF7 cell line. In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells—specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide-PEG-hydrazide, and subsequently anti-epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti-EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied in an Ephesia chip and an evaluation was performed by the MCF7 cell line.
dcterms:title
Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture
skos:prefLabel
Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture
skos:notation
RIV/61389013:_____/14:00427443!RIV15-AV0-61389013
n4:aktivita
n10:R n10:P n10:I
n4:aktivity
I, P(7E09109), R
n4:cisloPeriodika
2-3
n4:dodaniDat
n11:2015
n4:domaciTvurceVysledku
n6:6338135
n4:druhVysledku
n15:J
n4:duvernostUdaju
n8:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
3712
n4:idVysledku
RIV/61389013:_____/14:00427443
n4:jazykVysledku
n14:eng
n4:klicovaSlova
biofunctionalization; circulating tumor cells; EpCAM
n4:klicoveSlovo
n9:circulating%20tumor%20cells n9:biofunctionalization n9:EpCAM
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[8807ACC40BB9]
n4:nazevZdroje
Electrophoresis
n4:obor
n18:CD
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
7
n4:projekt
n16:7E09109
n4:rokUplatneniVysledku
n11:2014
n4:svazekPeriodika
35
n4:tvurceVysledku
Viovy, J.-L. Svobodová, Z. Bruckova, L. Autebert, J. Kučerová, J. Bílková, Z. Horák, Daniel
n4:wos
000331899400012
s:issn
0173-0835
s:numberOfPages
7
n13:doi
10.1002/elps.201300196