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Statements

Subject Item
n2:RIV%2F61389013%3A_____%2F13%3A00394385%21RIV14-MSM-61389013
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Maghemite (γ-Fe2O3) nanoparticles were prepared by coprecipitation of Fe(II) and Fe(III) salts followed by oxidation of Fe3O4 with sodium hypochlorite. The γ-Fe2O3 nanoparticles were then coated by a silica shell using tetramethyl orthosilicate producing γ-Fe2O3@SiO2 particles. In order to introduce amino groups, the surface of the γ-Fe2O3@SiO2 particles was coated with a second silica shell using (3-aminopropyl)triethoxysilane, resulting in γ-Fe2O3@SiO2-NH2 nanoparticles. The particles were characterized by scanning and transmission electron microscopy (SEM and TEM), dynamic light scattering (DLS) and Fourier-transform infrared (FT-IR) spectroscopy in terms of determination of morphology, particle size, polydispersity and presence of functional groups. Atomic absorption spectroscopy (AAS) was used for determination of the iron content. Moreover, induced magnetization and temperature-dependent magnetic susceptibility were investigated. In the biological experiments, γ-Fe2O3@SiO2 and γ-Fe2O3@SiO2-NH2 nanoparticles were incubated with murine macrophages of J774.2 line and the uptake of particles by the cells was visualized using both light and fluorescence microscopy. The silica surface coating did not affect the nanoparticle uptake by the cells, however, it decreased induced cytoplasmic vacuolization of macrophages. Maghemite (γ-Fe2O3) nanoparticles were prepared by coprecipitation of Fe(II) and Fe(III) salts followed by oxidation of Fe3O4 with sodium hypochlorite. The γ-Fe2O3 nanoparticles were then coated by a silica shell using tetramethyl orthosilicate producing γ-Fe2O3@SiO2 particles. In order to introduce amino groups, the surface of the γ-Fe2O3@SiO2 particles was coated with a second silica shell using (3-aminopropyl)triethoxysilane, resulting in γ-Fe2O3@SiO2-NH2 nanoparticles. The particles were characterized by scanning and transmission electron microscopy (SEM and TEM), dynamic light scattering (DLS) and Fourier-transform infrared (FT-IR) spectroscopy in terms of determination of morphology, particle size, polydispersity and presence of functional groups. Atomic absorption spectroscopy (AAS) was used for determination of the iron content. Moreover, induced magnetization and temperature-dependent magnetic susceptibility were investigated. In the biological experiments, γ-Fe2O3@SiO2 and γ-Fe2O3@SiO2-NH2 nanoparticles were incubated with murine macrophages of J774.2 line and the uptake of particles by the cells was visualized using both light and fluorescence microscopy. The silica surface coating did not affect the nanoparticle uptake by the cells, however, it decreased induced cytoplasmic vacuolization of macrophages.
dcterms:title
Silica-coated .gamma.-Fe2O3 nanoparticles: preparation and engulfment by mammalian macrophages Silica-coated .gamma.-Fe2O3 nanoparticles: preparation and engulfment by mammalian macrophages
skos:prefLabel
Silica-coated .gamma.-Fe2O3 nanoparticles: preparation and engulfment by mammalian macrophages Silica-coated .gamma.-Fe2O3 nanoparticles: preparation and engulfment by mammalian macrophages
skos:notation
RIV/61389013:_____/13:00394385!RIV14-MSM-61389013
n5:predkladatel
n9:ico%3A61389013
n3:aktivita
n13:I n13:P
n3:aktivity
I, P(7E12054), P(GCP207/12/J013)
n3:cisloPeriodika
2
n3:dodaniDat
n4:2014
n3:domaciTvurceVysledku
n6:6338135 Zasońska, Beata Anna n6:5920647
n3:druhVysledku
n18:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
105065
n3:idVysledku
RIV/61389013:_____/13:00394385
n3:jazykVysledku
n17:eng
n3:klicovaSlova
maghemite; nanoparticles; silica
n3:klicoveSlovo
n14:silica n14:maghemite n14:nanoparticles
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[AD3509AF958C]
n3:nazevZdroje
Journal of Nanopharmaceutics and Drug Delivery
n3:obor
n19:CD
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
7
n3:projekt
n15:GCP207%2F12%2FJ013 n15:7E12054
n3:rokUplatneniVysledku
n4:2013
n3:svazekPeriodika
1
n3:tvurceVysledku
Klyuchivska, O. Zasońska, Beata Anna Boiko, N. Trchová, Miroslava Horák, Daniel Stoika, R. Petrovský, Eduard
s:issn
2167-9312
s:numberOfPages
11
n10:doi
10.1166/jnd.2013.1020