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Statements

Subject Item
n2:RIV%2F61389013%3A_____%2F10%3A00341720%21RIV11-GA0-61389013
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. DLS, SLS, electrophoretic light scattering, SAXS, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30-60 nm. The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions. Polyplexes are polyelectrolyte complexes of DNA and polycations, designed for potential gene delivery. We investigated the properties of new polyplexes formed from cholesterol-modified polycations and DNA. Three complexes were tested; their cholesterol contents were 1.4, 6.3, and 8.7 mol %. UV spectroscopy and fluorescence assay using ethidium bromide proved the formation of polyplexes. The kinetics of turbidity of polyplexes solutions in physiological solution showed that the colloid stability of polyplexes increases with increasing content of cholesterol in polycations. DLS, SLS, electrophoretic light scattering, SAXS, and atomic force microscopy were used for characterization of polyplexes. The observed hydrodynamic radii of polyplexes were in the range of 30-60 nm. The properties of polyplex particles depend, in addition to polycation structure, on the rate of polycation addition to DNA solutions.
dcterms:title
Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes
skos:prefLabel
Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes Effect of hydrophobic interactions on properties and stability of DNA-polyelectrolyte complexes
skos:notation
RIV/61389013:_____/10:00341720!RIV11-GA0-61389013
n3:aktivita
n12:Z n12:P
n3:aktivity
P(GA202/09/2078), Z(AV0Z40500505)
n3:cisloPeriodika
7
n3:dodaniDat
n16:2011
n3:domaciTvurceVysledku
n13:1474545 n13:8924961 Filippov, Sergey n13:8168911 n13:9221948
n3:druhVysledku
n17:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
256018
n3:idVysledku
RIV/61389013:_____/10:00341720
n3:jazykVysledku
n4:eng
n3:klicovaSlova
DNA; polyplex; cholesterol
n3:klicoveSlovo
n5:polyplex n5:DNA n5:cholesterol
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[03F4DF85BAF3]
n3:nazevZdroje
Langmuir
n3:obor
n8:BO
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n14:GA202%2F09%2F2078
n3:rokUplatneniVysledku
n16:2010
n3:svazekPeriodika
26
n3:tvurceVysledku
Filippov, Sergey Štěpánek, Petr Koňák, Čestmír Kopečková, P. Starovoytova, Larisa Špírková, Milena
n3:wos
000275995100068
n3:zamer
n11:AV0Z40500505
s:issn
0743-7463
s:numberOfPages
8