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Statements

Subject Item
n2:RIV%2F61389005%3A_____%2F03%3A49033071%21RIV%2F2004%2FAV0%2FA49004%2FN
rdf:type
skos:Concept n15:Vysledek
dcterms:description
We have investigated a method for direct astatine labeling of proteins. Binding sites for astatine were created by coupling of a nido-carborane derivative to a protein, the human epidermal growth factor (hEGF), using two different conjugation methods - by glutaraldehyde cross-linking or by introduction of sulfohydryl groups by Traut's reagent with subsequent linking of ANC-1 with m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester. The conjugates were astatinated using the Chloramine-T method in high yield. The best labeling was obtained by the glutaraldehyde conjugate with an average yield of 68+/-9%. In vitro stability tests indicated that the glutaraldehyde conjugated label was as stable as hEGF labeled with astatobenzoate. We have investigated a method for direct astatine labeling of proteins. Binding sites for astatine were created by coupling of a nido-carborane derivative to a protein, the human epidermal growth factor (hEGF), using two different conjugation methods - by glutaraldehyde cross-linking or by introduction of sulfohydryl groups by Traut's reagent with subsequent linking of ANC-1 with m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester. The conjugates were astatinated using the Chloramine-T method in high yield. The best labeling was obtained by the glutaraldehyde conjugate with an average yield of 68+/-9%. In vitro stability tests indicated that the glutaraldehyde conjugated label was as stable as hEGF labeled with astatobenzoate.
dcterms:title
Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group. Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group.
skos:prefLabel
Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group. Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group.
skos:notation
RIV/61389005:_____/03:49033071!RIV/2004/AV0/A49004/N
n3:strany
191;197
n3:aktivita
n9:P
n3:aktivity
P(KSK4055109)
n3:cisloPeriodika
7
n3:dodaniDat
n5:2004
n3:domaciTvurceVysledku
n10:7639821
n3:druhVysledku
n7:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
603999
n3:idVysledku
RIV/61389005:_____/03:49033071
n3:jazykVysledku
n14:eng
n3:klicovaSlova
neutron-capture therapy; astatine-211
n3:klicoveSlovo
n6:neutron-capture%20therapy n6:astatine-211
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[C96A41941603]
n3:nazevZdroje
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
n3:obor
n11:CH
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n16:KSK4055109
n3:rokUplatneniVysledku
n5:2003
n3:svazekPeriodika
256
n3:tvurceVysledku
Koziorowski, J. Lebeda, Ondřej Sjostrom, A. Lundqvist, H. Tolmachev, V. Carlsson, J.
s:issn
0236-5731
s:numberOfPages
7