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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F14%3A00433025%21RIV15-GA0-61388971
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Rhodococcus erythropolis CCM2595 is able to efficiently utilize phenol and other aromatic compounds. We cloned and sequenced its complete gene cluster - catA, catB, catC, catR, pheR, pheA2, pheA1 - involved in the ortho-cleavage pathway of phenol. The activity of the key enzyme of the phenol degradation pathway, two-component phenol hydroxylase, was found to be induced by phenol. When both phenol and succinate were present in the medium, phenol hydroxylase activity decreased substantially. To analyze the regulation of phenol degradation at the transcriptional level, the transcriptional fusions of the divergently oriented promoters PpheA2 and PpheR with the gfpuv reporter gene were constructed. The promoters driving expression of the genes of the pheR-pheA2pheA1 cluster were localized by determining the respective transcriptional start points Rhodococcus erythropolis CCM2595 is able to efficiently utilize phenol and other aromatic compounds. We cloned and sequenced its complete gene cluster - catA, catB, catC, catR, pheR, pheA2, pheA1 - involved in the ortho-cleavage pathway of phenol. The activity of the key enzyme of the phenol degradation pathway, two-component phenol hydroxylase, was found to be induced by phenol. When both phenol and succinate were present in the medium, phenol hydroxylase activity decreased substantially. To analyze the regulation of phenol degradation at the transcriptional level, the transcriptional fusions of the divergently oriented promoters PpheA2 and PpheR with the gfpuv reporter gene were constructed. The promoters driving expression of the genes of the pheR-pheA2pheA1 cluster were localized by determining the respective transcriptional start points
dcterms:title
Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis nad Rhodococcus jostii Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis nad Rhodococcus jostii
skos:prefLabel
Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis nad Rhodococcus jostii Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis nad Rhodococcus jostii
skos:notation
RIV/61388971:_____/14:00433025!RIV15-GA0-61388971
n3:aktivita
n13:P n13:I
n3:aktivity
I, P(2B08062), P(GAP504/11/0394)
n3:cisloPeriodika
19
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n4:5053110 n4:9197877 n4:6672922 n4:2185148 n4:2343835
n3:druhVysledku
n9:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
21232
n3:idVysledku
RIV/61388971:_____/14:00433025
n3:jazykVysledku
n5:eng
n3:klicovaSlova
Phenol degradation; Phenol hydroxylase; Rhodococcus erythropolis
n3:klicoveSlovo
n12:Rhodococcus%20erythropolis n12:Phenol%20degradation n12:Phenol%20hydroxylase
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[1A682A416B8B]
n3:nazevZdroje
Applied Microbiology and Biotechnology
n3:obor
n16:EE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n7:2B08062 n7:GAP504%2F11%2F0394
n3:rokUplatneniVysledku
n10:2014
n3:svazekPeriodika
98
n3:tvurceVysledku
Szököl, Juraj Halada, Petr Rucká, Lenka Nešvera, Jan Šimčíková, M. Pátek, Miroslav
n3:wos
000342072500018
s:issn
0175-7598
s:numberOfPages
12
n11:doi
10.1007/s00253-014-5881-6