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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F13%3A00425117%21RIV14-MSM-61388971
rdf:type
n8:Vysledek skos:Concept
dcterms:description
The determination of phycobilisome diffusivity (diffusion coefficient D) on thylakoid membrane from fluorescence recovery after photobleaching (FRAP) experiments is usually done by analytical models. However, analytical models need some unrealistic conditions to be supposed. This study describes the development of a method based on finite difference approximation of the process governed by the Fickian diffusion equation and on the minimization of an objective function representing the disparity between the experimental and simulated time-varying concentration profiles. Our method improves on other models by accounting for experimentally measured time-varying Dirichlet boundary conditions, and can include a reaction term as well. As a result we obtain both the overall (time averaged) diffusion coefficient D and the sequence of diffusivities Dj based on two successive fluorescence profiles in j-th time interval. Due to the noisy data, we cope with an inverse ill-posed problem and a regularization technique is mandatory. The determination of phycobilisome diffusivity (diffusion coefficient D) on thylakoid membrane from fluorescence recovery after photobleaching (FRAP) experiments is usually done by analytical models. However, analytical models need some unrealistic conditions to be supposed. This study describes the development of a method based on finite difference approximation of the process governed by the Fickian diffusion equation and on the minimization of an objective function representing the disparity between the experimental and simulated time-varying concentration profiles. Our method improves on other models by accounting for experimentally measured time-varying Dirichlet boundary conditions, and can include a reaction term as well. As a result we obtain both the overall (time averaged) diffusion coefficient D and the sequence of diffusivities Dj based on two successive fluorescence profiles in j-th time interval. Due to the noisy data, we cope with an inverse ill-posed problem and a regularization technique is mandatory.
dcterms:title
Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images
skos:prefLabel
Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images
skos:notation
RIV/61388971:_____/13:00425117!RIV14-MSM-61388971
n8:predkladatel
n18:ico%3A61388971
n3:aktivita
n12:Z n12:P
n3:aktivity
P(ED2.1.00/01.0024), P(ED2.1.00/03.0110), P(GA201/09/1957), P(GP206/09/P094), Z(AV0Z10300504), Z(AV0Z50200510)
n3:cisloPeriodika
7-8
n3:dodaniDat
n6:2014
n3:domaciTvurceVysledku
n20:6355897
n3:druhVysledku
n15:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
73268
n3:idVysledku
RIV/61388971:_____/13:00425117
n3:jazykVysledku
n19:eng
n3:klicovaSlova
parameter estimation; FRAP; boundary value problem; optimization
n3:klicoveSlovo
n7:boundary%20value%20problem n7:FRAP n7:optimization n7:parameter%20estimation
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[6B8D9D7FDF8E]
n3:nazevZdroje
Mathematical and Computer Modelling
n3:obor
n13:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:projekt
n16:GA201%2F09%2F1957 n16:ED2.1.00%2F03.0110 n16:ED2.1.00%2F01.0024 n16:GP206%2F09%2FP094
n3:rokUplatneniVysledku
n6:2013
n3:svazekPeriodika
57
n3:tvurceVysledku
Papáček, Š. Matonoha, Ctirad Kaňa, Radek
n3:wos
000315863300045
n3:zamer
n4:AV0Z50200510 n4:AV0Z10300504
s:issn
0895-7177
s:numberOfPages
6
n11:doi
10.1016/j.mcm.2011.12.029