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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F13%3A00423803%21RIV14-MSM-61388971
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Fungi are important in soils as both decomposers and plant symbionts, and an understanding of the composition of their complex communities is thus indispensable to answer a variety of ecological questions. 454 Pyrosequencing is currently the method of choice for the in-depth analysis of fungal communities. However, the interpretation of its results is complicated by differences in data analysis approaches that make inter-study comparisons difficult. The pyrosequencing studies published so far have also used variable molecular targets in fungal rDNA. Although the ITS region and, in particular, ITS1 appear to be the most frequent sequencing targets, the use of various primers with different coverages of fungal groups remains a serious problem. Sequence length limits also vary widely across studies, and in many studies, length differences may negatively affect sequence similarity clustering or identification Fungi are important in soils as both decomposers and plant symbionts, and an understanding of the composition of their complex communities is thus indispensable to answer a variety of ecological questions. 454 Pyrosequencing is currently the method of choice for the in-depth analysis of fungal communities. However, the interpretation of its results is complicated by differences in data analysis approaches that make inter-study comparisons difficult. The pyrosequencing studies published so far have also used variable molecular targets in fungal rDNA. Although the ITS region and, in particular, ITS1 appear to be the most frequent sequencing targets, the use of various primers with different coverages of fungal groups remains a serious problem. Sequence length limits also vary widely across studies, and in many studies, length differences may negatively affect sequence similarity clustering or identification
dcterms:title
Analysis of soil fungal communities by amplicon pyrosequencing: current approaches to data analysis and the introduction of the pipeline SEED Analysis of soil fungal communities by amplicon pyrosequencing: current approaches to data analysis and the introduction of the pipeline SEED
skos:prefLabel
Analysis of soil fungal communities by amplicon pyrosequencing: current approaches to data analysis and the introduction of the pipeline SEED Analysis of soil fungal communities by amplicon pyrosequencing: current approaches to data analysis and the introduction of the pipeline SEED
skos:notation
RIV/61388971:_____/13:00423803!RIV14-MSM-61388971
n15:predkladatel
n16:ico%3A61388971
n5:aktivita
n14:P n14:I
n5:aktivity
I, P(GAP504/12/0709), P(LD12048), P(LD12050)
n5:cisloPeriodika
8
n5:dodaniDat
n7:2014
n5:domaciTvurceVysledku
n13:4747305 n13:7305834
n5:druhVysledku
n9:J
n5:duvernostUdaju
n18:S
n5:entitaPredkladatele
n17:predkladatel
n5:idSjednocenehoVysledku
60639
n5:idVysledku
RIV/61388971:_____/13:00423803
n5:jazykVysledku
n6:eng
n5:klicovaSlova
Fungal community; Internal transcribed spacer; Pyrosequencing pipeline
n5:klicoveSlovo
n8:Fungal%20community n8:Internal%20transcribed%20spacer n8:Pyrosequencing%20pipeline
n5:kodStatuVydavatele
NL - Nizozemsko
n5:kontrolniKodProRIV
[84772FE6A898]
n5:nazevZdroje
Biology and Fertility of Soils
n5:obor
n10:EE
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
2
n5:projekt
n12:GAP504%2F12%2F0709 n12:LD12048 n12:LD12050
n5:rokUplatneniVysledku
n7:2013
n5:svazekPeriodika
49
n5:tvurceVysledku
Baldrian, Petr Větrovský, Tomáš
n5:wos
000326374900005
s:issn
0178-2762
s:numberOfPages
11
n19:doi
10.1007/s00374-013-0801-y