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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F13%3A00422632%21RIV14-MSM-61388971
rdf:type
skos:Concept n10:Vysledek
dcterms:description
This study describes an efficient, large scale fermentation of a recombinant alpha-L-rhamnosidase originating from Aspergillus terreus. High-cell-density Pichia pastoris fermentation resulted in yields up to 627 U/L/h. The recombinant enzyme was used for the reverse rhamnosylation of various small organic compounds. A full factorial experimental design setup was applied to identify the importance of temperature, substrate concentrations, solvent type and concentration as well as the acidity of the reaction mixture. Careful optimization of these parameters allowed the synthesis of a range of alpha-L-rhamnosides among which cyclohexyl alpha-L-rhamnopyranoside, anisyl alpha-L-rhamnopyranoside and 2-phenylethyl alpha-L-rhamnopyranoside. In addition, alpha-L-rhamnosylation of phenolic hydroxyls in phenols such as hydroquinone, resorcinol, catechol and phenol was observed, which is a rather unique reaction catalyzed by glycosidases This study describes an efficient, large scale fermentation of a recombinant alpha-L-rhamnosidase originating from Aspergillus terreus. High-cell-density Pichia pastoris fermentation resulted in yields up to 627 U/L/h. The recombinant enzyme was used for the reverse rhamnosylation of various small organic compounds. A full factorial experimental design setup was applied to identify the importance of temperature, substrate concentrations, solvent type and concentration as well as the acidity of the reaction mixture. Careful optimization of these parameters allowed the synthesis of a range of alpha-L-rhamnosides among which cyclohexyl alpha-L-rhamnopyranoside, anisyl alpha-L-rhamnopyranoside and 2-phenylethyl alpha-L-rhamnopyranoside. In addition, alpha-L-rhamnosylation of phenolic hydroxyls in phenols such as hydroquinone, resorcinol, catechol and phenol was observed, which is a rather unique reaction catalyzed by glycosidases
dcterms:title
Chemoenzymatic synthesis of alpha-L-rhamnosides using recombinant alpha-L-rhamnosidase from Aspergillus terreus Chemoenzymatic synthesis of alpha-L-rhamnosides using recombinant alpha-L-rhamnosidase from Aspergillus terreus
skos:prefLabel
Chemoenzymatic synthesis of alpha-L-rhamnosides using recombinant alpha-L-rhamnosidase from Aspergillus terreus Chemoenzymatic synthesis of alpha-L-rhamnosides using recombinant alpha-L-rhamnosidase from Aspergillus terreus
skos:notation
RIV/61388971:_____/13:00422632!RIV14-MSM-61388971
n10:predkladatel
n18:ico%3A61388971
n3:aktivita
n7:P n7:I
n3:aktivity
I, P(7E11011), P(LD13042)
n3:cisloPeriodika
NOV 2013
n3:dodaniDat
n14:2014
n3:domaciTvurceVysledku
n4:4878922 n4:5894190 n4:7346689 n4:6883753
n3:druhVysledku
n19:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
65248
n3:idVysledku
RIV/61388971:_____/13:00422632
n3:jazykVysledku
n17:eng
n3:klicovaSlova
alpha-L-Rhamnosidase; Aspergillus terreus; Glycosylation
n3:klicoveSlovo
n9:Glycosylation n9:Aspergillus%20terreus n9:alpha-L-Rhamnosidase
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[7FF8B770FDBD]
n3:nazevZdroje
Bioresource Technology
n3:obor
n13:CE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
8
n3:projekt
n12:7E11011 n12:LD13042
n3:rokUplatneniVysledku
n14:2013
n3:svazekPeriodika
147
n3:tvurceVysledku
Soetaert, W. Desmet, T. Pelantová, Helena Křen, Vladimír Šimčíková, Daniela Weignerová, Lenka De Winter, K. Schlack, B.
n3:wos
000326660900088
s:issn
0960-8524
s:numberOfPages
5
n16:doi
10.1016/j.biortech.2013.08.083