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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F13%3A00399622%21RIV14-AV0-61388971
rdf:type
skos:Concept n12:Vysledek
dcterms:description
IL-2/anti-IL-2 mAb immunocomplexes were described to have dramatically higher activity than free IL-2 in vivo. We designed protein chimera consisting of IL-2 linked to light chain of anti-IL-2 mAb S4B6 through flexible oligopeptide spacer (Gly(4)Ser)(3). This protein chimera mimics the structure of IL-2/S4B6 mAb immunocomplexes but eliminates general disadvantages of immunocomplexes like possible excess of either IL-2 or anti-IL-2 mAb and their dissociation to antibody and IL-2 at low concentrations. This novel kind of protein chimera is characterized by an intramolecular interaction between IL-2 and binding site of S4B6 mAb similarly as in IL-2/S4B6 mAb immunocomplexes. Our protein chimera has biological activity comparable to IL-2/S4B6 mAb immunocomplexes in vitro, as shown by stimulation of proliferation of purified and activated OT-I CD8(+) T cells. The protein chimera exerts higher stimulatory activity to drive expansion of purified CFSE-labeled OT-I CD8(+) T cells activated by an injection of a low dose of SIINFEKL peptide than IL-2/S4B6 mAb immunocomplexes in vivo IL-2/anti-IL-2 mAb immunocomplexes were described to have dramatically higher activity than free IL-2 in vivo. We designed protein chimera consisting of IL-2 linked to light chain of anti-IL-2 mAb S4B6 through flexible oligopeptide spacer (Gly(4)Ser)(3). This protein chimera mimics the structure of IL-2/S4B6 mAb immunocomplexes but eliminates general disadvantages of immunocomplexes like possible excess of either IL-2 or anti-IL-2 mAb and their dissociation to antibody and IL-2 at low concentrations. This novel kind of protein chimera is characterized by an intramolecular interaction between IL-2 and binding site of S4B6 mAb similarly as in IL-2/S4B6 mAb immunocomplexes. Our protein chimera has biological activity comparable to IL-2/S4B6 mAb immunocomplexes in vitro, as shown by stimulation of proliferation of purified and activated OT-I CD8(+) T cells. The protein chimera exerts higher stimulatory activity to drive expansion of purified CFSE-labeled OT-I CD8(+) T cells activated by an injection of a low dose of SIINFEKL peptide than IL-2/S4B6 mAb immunocomplexes in vivo
dcterms:title
Chimera of IL-2 Linked to Light Chain of anti-IL-2 mAb Mimics IL-2/anti-IL-2 mAb Complexes Both Structurally and Functionally Chimera of IL-2 Linked to Light Chain of anti-IL-2 mAb Mimics IL-2/anti-IL-2 mAb Complexes Both Structurally and Functionally
skos:prefLabel
Chimera of IL-2 Linked to Light Chain of anti-IL-2 mAb Mimics IL-2/anti-IL-2 mAb Complexes Both Structurally and Functionally Chimera of IL-2 Linked to Light Chain of anti-IL-2 mAb Mimics IL-2/anti-IL-2 mAb Complexes Both Structurally and Functionally
skos:notation
RIV/61388971:_____/13:00399622!RIV14-AV0-61388971
n12:predkladatel
n15:ico%3A61388971
n5:aktivita
n17:P n17:I
n5:aktivity
I, P(GAP301/11/0325)
n5:cisloPeriodika
5
n5:dodaniDat
n9:2014
n5:domaciTvurceVysledku
n8:4183746 n8:3220206 n8:5287944 n8:6598099 n8:2280906 n8:9165614 n8:3814556 n8:1130706
n5:druhVysledku
n18:J
n5:duvernostUdaju
n13:S
n5:entitaPredkladatele
n16:predkladatel
n5:idSjednocenehoVysledku
65279
n5:idVysledku
RIV/61388971:_____/13:00399622
n5:jazykVysledku
n19:eng
n5:klicovaSlova
IN-VIVO EXPANSION; IL-2; ANTIBODY
n5:klicoveSlovo
n11:ANTIBODY n11:IN-VIVO%20EXPANSION n11:IL-2
n5:kodStatuVydavatele
US - Spojené státy americké
n5:kontrolniKodProRIV
[A0B041700FB0]
n5:nazevZdroje
ACS Chemical Biology
n5:obor
n6:CE
n5:pocetDomacichTvurcuVysledku
8
n5:pocetTvurcuVysledku
8
n5:projekt
n7:GAP301%2F11%2F0325
n5:rokUplatneniVysledku
n9:2013
n5:svazekPeriodika
8
n5:tvurceVysledku
Kovářová, Jiřina Dvořáková, Barbora Votavová, Petra Chmelová, Helena Kovář, Marek Tomala, Jakub Kabešová, Martina Říhová, Blanka
n5:wos
000319720700003
s:issn
1554-8929
s:numberOfPages
6
n14:doi
10.1021/cb3007242