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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F13%3A00399060%21RIV14-AV0-61388971
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Nitrilases are highly conserved proteins with catabolic activity but much less understood functions in cell division and apoptosis. To elucidate the biological functions of Arabidopsis NITRILASE1, we characterized its molecular forms, cellular localization and involvement in cell proliferation and plant development. We performed biochemical and mass spectrometry analyses of NITRILASE1 complexes, electron microscopy of nitrilase polymers, imaging of developmental and cellular distribution, silencing and overexpression of nitrilases to study their functions. We found that NITRILASE1 has an intrinsic ability to form filaments. GFP-NITRILASE1 was abundant in proliferating cells, distributed in cytoplasm, in the perinuclear area and associated with microtubules. As cells exited proliferation and entered differentiation, GFP-NITRILASE1 became predominantly nuclear. Nitrilase silencing dose-dependently compromised plant growth, led to loss of tissue organization and sustained proliferation. Cytokinesis was frequently aborted, leading to enlarged polyploid cells. In reverse, independently transformed cell lines overexpressing GFP-NITRILASE1 showed slow growth and increased rate of programmed cell death. Altogether, our data suggest that NITRILASE1 homologues regulate the exit from cell cycle and entry into differentiation and simultaneously are required for cytokinesis. These functions are essential to maintain normal ploidy, genome stability and tissue organization Nitrilases are highly conserved proteins with catabolic activity but much less understood functions in cell division and apoptosis. To elucidate the biological functions of Arabidopsis NITRILASE1, we characterized its molecular forms, cellular localization and involvement in cell proliferation and plant development. We performed biochemical and mass spectrometry analyses of NITRILASE1 complexes, electron microscopy of nitrilase polymers, imaging of developmental and cellular distribution, silencing and overexpression of nitrilases to study their functions. We found that NITRILASE1 has an intrinsic ability to form filaments. GFP-NITRILASE1 was abundant in proliferating cells, distributed in cytoplasm, in the perinuclear area and associated with microtubules. As cells exited proliferation and entered differentiation, GFP-NITRILASE1 became predominantly nuclear. Nitrilase silencing dose-dependently compromised plant growth, led to loss of tissue organization and sustained proliferation. Cytokinesis was frequently aborted, leading to enlarged polyploid cells. In reverse, independently transformed cell lines overexpressing GFP-NITRILASE1 showed slow growth and increased rate of programmed cell death. Altogether, our data suggest that NITRILASE1 homologues regulate the exit from cell cycle and entry into differentiation and simultaneously are required for cytokinesis. These functions are essential to maintain normal ploidy, genome stability and tissue organization
dcterms:title
NITRILASE1 regulates the exit from proliferation, genome stability and plant development NITRILASE1 regulates the exit from proliferation, genome stability and plant development
skos:prefLabel
NITRILASE1 regulates the exit from proliferation, genome stability and plant development NITRILASE1 regulates the exit from proliferation, genome stability and plant development
skos:notation
RIV/61388971:_____/13:00399060!RIV14-AV0-61388971
n10:predkladatel
n11:ico%3A61388971
n3:aktivita
n5:P n5:I
n3:aktivity
I, P(GAP501/12/2333)
n3:cisloPeriodika
3
n3:dodaniDat
n12:2014
n3:domaciTvurceVysledku
n4:2924056 n4:8652287 n4:1514024 n4:2185148 n4:1848097 n4:5791006 n4:5386403 n4:6282520 n4:9167838
n3:druhVysledku
n19:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
91819
n3:idVysledku
RIV/61388971:_____/13:00399060
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Arabidopsis; cell cycle; cytokinesis
n3:klicoveSlovo
n14:cell%20cycle n14:Arabidopsis n14:cytokinesis
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[16B7623BCF5A]
n3:nazevZdroje
New Phytologist
n3:obor
n17:EE
n3:pocetDomacichTvurcuVysledku
9
n3:pocetTvurcuVysledku
11
n3:projekt
n9:GAP501%2F12%2F2333
n3:rokUplatneniVysledku
n12:2013
n3:svazekPeriodika
198
n3:tvurceVysledku
Petrovská, Beáta Plíhal, Ondřej Bögre, L. Halada, Petr Kohoutová, Lucie Doskočilová, Anna Volc, Jindřich Binarová, Pavla Benada, Oldřich Kourová, Hana Chumová, Jana
n3:wos
000317682900009
s:issn
0028-646X
s:numberOfPages
14
n13:doi
10.1111/nph.12185