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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F13%3A00393101%21RIV14-MSM-61388971
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Aims To perform a systematic evaluation of the applicability, validity and reliability of the long PCR-RFLP of 16S-ITS-23S rRNA genes for bacterial genotyping using both sequences retrieved from public genome databases and the experimental data obtained on bacterial cultures. Methods and Results 3301 Full-length sequences of 16S-ITS-23S rRNA genes were retrieved from 885 published bacterial genomes. Copy numbers of the whole set of 16S-ITS-23S rRNA genes per genome ranged from 1 (n = 161) to 14 (n = 4) with an average of 3.71. Their length varied greatly, from 4319 to 6568 bp with an average of 4952 bp. Computer-simulated RFLP analyses of the 16S-ITS-23S fragments flanked by the conserved primers 27F and 2241R suggested MspI, RsaI, HhaI and TaqI as the most appropriate enzymes for long PCR-RFLP analysis of the 16S-ITS-23S sequence Aims To perform a systematic evaluation of the applicability, validity and reliability of the long PCR-RFLP of 16S-ITS-23S rRNA genes for bacterial genotyping using both sequences retrieved from public genome databases and the experimental data obtained on bacterial cultures. Methods and Results 3301 Full-length sequences of 16S-ITS-23S rRNA genes were retrieved from 885 published bacterial genomes. Copy numbers of the whole set of 16S-ITS-23S rRNA genes per genome ranged from 1 (n = 161) to 14 (n = 4) with an average of 3.71. Their length varied greatly, from 4319 to 6568 bp with an average of 4952 bp. Computer-simulated RFLP analyses of the 16S-ITS-23S fragments flanked by the conserved primers 27F and 2241R suggested MspI, RsaI, HhaI and TaqI as the most appropriate enzymes for long PCR-RFLP analysis of the 16S-ITS-23S sequence
dcterms:title
Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping
skos:prefLabel
Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping
skos:notation
RIV/61388971:_____/13:00393101!RIV14-MSM-61388971
n16:predkladatel
n17:ico%3A61388971
n3:aktivita
n9:P n9:I
n3:aktivity
I, P(ED2.1.00/03.0110)
n3:cisloPeriodika
2
n3:dodaniDat
n4:2014
n3:domaciTvurceVysledku
Zeng, Yonghui n14:4761766
n3:druhVysledku
n7:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
85350
n3:idVysledku
RIV/61388971:_____/13:00393101
n3:jazykVysledku
n19:eng
n3:klicovaSlova
16S-ITS-23S; bacterial genome; computer simulation
n3:klicoveSlovo
n6:computer%20simulation n6:16S-ITS-23S n6:bacterial%20genome
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[9DC767472CD1]
n3:nazevZdroje
Journal of Applied Microbiology
n3:obor
n15:EE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
8
n3:projekt
n18:ED2.1.00%2F03.0110
n3:rokUplatneniVysledku
n4:2013
n3:svazekPeriodika
114
n3:tvurceVysledku
Feng, F. Y. Wu, Z. H. Ji, J. D. Li, Y. X. Liu, Y. P. Koblížek, Michal Zeng, Yonghui Jian, J. C.
n3:wos
000313723800015
s:issn
1364-5072
s:numberOfPages
15
n12:doi
10.1111/jam.12057