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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F12%3A00378997%21RIV13-GA0-61388971
rdf:type
skos:Concept n10:Vysledek
dcterms:description
The Bordetella adenylate cyclase toxin-hemolysin (CyaA; also called ACT or AC-Hly) targets CD11b-expressing phagocytes and translocates into their cytosol an adenylyl cyclase (AC) that hijacks cellular signaling by conversion of ATP to cyclic AMP (cAMP). Intriguingly, insertion of large passenger peptides removes the enzymatic activity but not the cell-invasive capacity of the AC domain. This has repeatedly been exploited for delivery of heterologous antigens into the cytosolic pathway of CD11b-expressing dendritic cells by CyaA/AC(-) toxoids, thus enabling their processing and presentation on major histocompatibility complex (MHC) class I molecules to cytotoxic CD8(+) T lymphocytes (CTLs). We produced a set of toxoids with overlapping deletions within the first 371 residues of CyaA and showed that the structure of the AC enzyme does not contain any sequences indispensable for its translocation across target cell membrane The Bordetella adenylate cyclase toxin-hemolysin (CyaA; also called ACT or AC-Hly) targets CD11b-expressing phagocytes and translocates into their cytosol an adenylyl cyclase (AC) that hijacks cellular signaling by conversion of ATP to cyclic AMP (cAMP). Intriguingly, insertion of large passenger peptides removes the enzymatic activity but not the cell-invasive capacity of the AC domain. This has repeatedly been exploited for delivery of heterologous antigens into the cytosolic pathway of CD11b-expressing dendritic cells by CyaA/AC(-) toxoids, thus enabling their processing and presentation on major histocompatibility complex (MHC) class I molecules to cytotoxic CD8(+) T lymphocytes (CTLs). We produced a set of toxoids with overlapping deletions within the first 371 residues of CyaA and showed that the structure of the AC enzyme does not contain any sequences indispensable for its translocation across target cell membrane
dcterms:title
Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain
skos:prefLabel
Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain Delivery of Large Heterologous Polypeptides across the Cytoplasmic Membrane of Antigen-Presenting Cells by the Bordetella RTX Hemolysin Moiety Lacking the Adenylyl Cyclase Domain
skos:notation
RIV/61388971:_____/12:00378997!RIV13-GA0-61388971
n10:predkladatel
n11:ico%3A61388971
n3:aktivita
n7:Z n7:P
n3:aktivity
P(1M0506), P(2B06161), P(GAP207/11/0717), P(GAP301/11/0325), P(IAA500200914), Z(AV0Z50200510)
n3:cisloPeriodika
3
n3:dodaniDat
n8:2013
n3:domaciTvurceVysledku
n5:5476054 n5:9638571 n5:1076329 n5:3814556 n5:2889528 n5:5075084 n5:1701444 n5:5377846 n5:5287944
n3:druhVysledku
n13:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
129984
n3:idVysledku
RIV/61388971:_____/12:00378997
n3:jazykVysledku
n20:eng
n3:klicovaSlova
MHC CLASS-I; ESCHERICHIA-COLI; PRESENTATION PATHWAY
n3:klicoveSlovo
n9:MHC%20CLASS-I n9:ESCHERICHIA-COLI n9:PRESENTATION%20PATHWAY
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[B75B6E49C098]
n3:nazevZdroje
Infection and Immunity
n3:obor
n19:EE
n3:pocetDomacichTvurcuVysledku
9
n3:pocetTvurcuVysledku
11
n3:projekt
n4:GAP207%2F11%2F0717 n4:GAP301%2F11%2F0325 n4:1M0506 n4:IAA500200914 n4:2B06161
n3:rokUplatneniVysledku
n8:2012
n3:svazekPeriodika
80
n3:tvurceVysledku
Michálek, J. Bumba, Ladislav Tomala, Jakub Kamanová, Jana Staněk, Ondřej Kosová, Martina Kovář, Marek Jelínek, J. Mašín, Jiří Holubová, Jana Šebo, Peter
n3:wos
000300621600031
n3:zamer
n14:AV0Z50200510
s:issn
0019-9567
s:numberOfPages
12
n18:doi
10.1128/IAI.05711-11