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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F11%3A00369717%21RIV12-AV0-61388971
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple and efficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple and efficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain
dcterms:title
High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli
skos:prefLabel
High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli
skos:notation
RIV/61388971:_____/11:00369717!RIV12-AV0-61388971
n13:predkladatel
n20:ico%3A61388971
n3:aktivita
n4:S n4:P n4:Z
n3:aktivity
P(1M0505), P(GA303/09/0477), P(GAP207/10/1040), P(GD305/09/H008), P(IAA500200620), P(LC07017), P(LC545), S, Z(AV0Z50200510), Z(MSM0021620808)
n3:cisloPeriodika
2
n3:dodaniDat
n14:2012
n3:domaciTvurceVysledku
n9:8710740 n9:1346946 n9:8732124 n9:8652406 n9:7750161 n9:2100592
n3:druhVysledku
n18:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
202069
n3:idVysledku
RIV/61388971:_____/11:00369717
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Natural killer cell; NKR-P1C receptor; NK1.1 antigen
n3:klicoveSlovo
n15:Natural%20killer%20cell n15:NK1.1%20antigen n15:NKR-P1C%20receptor
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[42B1EBF70FE3]
n3:nazevZdroje
Protein Expression and Purification
n3:obor
n17:EC
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n7:GA303%2F09%2F0477 n7:GAP207%2F10%2F1040 n7:GD305%2F09%2FH008 n7:IAA500200620 n7:LC545 n7:1M0505 n7:LC07017
n3:rokUplatneniVysledku
n14:2011
n3:svazekPeriodika
77
n3:tvurceVysledku
Vaněk, Ondřej Bezouška, Karel Kavan, Daniel Rozbeský, Daniel Chmelík, Josef Novák, Petr
n3:wos
000288591900008
n3:zamer
n5:MSM0021620808 n5:AV0Z50200510
s:issn
1046-5928
s:numberOfPages
7
n19:doi
10.1016/j.pep.2011.01.013