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Statements

Subject Item
n2:RIV%2F61388971%3A_____%2F11%3A00360791%21RIV12-AV0-61388971
rdf:type
n7:Vysledek skos:Concept
dcterms:description
Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize omega-alicyclic fatty acids (omega-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize omega-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FA composition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of omega-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of omega-FAs not found in the natural environment of Geobacillus and Meiothermus strains Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize omega-alicyclic fatty acids (omega-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize omega-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FA composition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of omega-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of omega-FAs not found in the natural environment of Geobacillus and Meiothermus strains
dcterms:title
Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber
skos:prefLabel
Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber
skos:notation
RIV/61388971:_____/11:00360791!RIV12-AV0-61388971
n7:predkladatel
n8:ico%3A61388971
n3:aktivita
n6:Z
n3:aktivity
Z(AV0Z50200510)
n3:cisloPeriodika
3
n3:dodaniDat
n4:2012
n3:domaciTvurceVysledku
n15:6536239 n15:8377898
n3:druhVysledku
n13:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
188439
n3:idVysledku
RIV/61388971:_____/11:00360791
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Thermophilic bacteria; Geobacillus; Meiothermus
n3:klicoveSlovo
n14:Meiothermus n14:Geobacillus n14:Thermophilic%20bacteria
n3:kodStatuVydavatele
JP - Japonsko
n3:kontrolniKodProRIV
[349384CBFB47]
n3:nazevZdroje
Extremophiles
n3:obor
n19:EE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n4:2011
n3:svazekPeriodika
15
n3:tvurceVysledku
Luhový, R. Sigler, Karel Siřišťová, L. Řezanka, Tomáš
n3:wos
000290037700012
n3:zamer
n12:AV0Z50200510
s:issn
1431-0651
s:numberOfPages
7
n16:doi
10.1007/s00792-011-0373-4